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. 1986 Dec 24;95(2):273-6.
doi: 10.1016/0022-1759(86)90415-1.

Purification of human monocytes on gelatin-coated surfaces

N F Hassan, D E Campbell, S D Douglas

Purification of human monocytes on gelatin-coated surfaces

N F Hassan et al. J Immunol Methods. .

Abstract

Human peripheral blood monocytes secrete a cell membrane-associated glycoprotein, cold insoluble globulin (fibronectin). Since fibronectin binds to gelatin-coated surface, we developed a simple technique for separation of human peripheral blood monocytes from whole mononuclear cell preparation. These preparations are characterized by a high monocyte purity (more than 90%), low platelet contamination and excellent viability.

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