DNA flow cytometry of histological material from dysplastic lesions of human gastric mucosa

Abstract

DNA flow cytometry has been carried out on histologically processed human gastric tissue showing varying grades of gastric dysplasia, intramucosal carcinoma and regenerative changes. No cases of DNA aneuploidy were encountered in cases showing regenerative changes or mild or moderate dysplasia. DNA aneuploidy was found in 5/7 cases of severe dysplasia and 3/11 cases of intramucosal cancer. Irregularities of the G0/G1 peak were encountered in a further 18 samples including normal and regenerative gastric mucosa. However these were not thought to indicate DNA aneuploidy. The interpretation of these abnormalities and the affect on the frequency with which DNA aneuploidy is reported in pathological lesions is discussed.