Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Nov 14;23(1):558.
doi: 10.1186/s12870-023-04595-z.

Regulatory effects of Hemin on prevention and rescue of salt stress in rapeseed (Brassica napus L.) seedlings

Hui-Min Zhao  1   2 , Dian-Feng Zheng  3   4   5 , Nai-Jie Feng  6   7   8 , Guang-Sheng Zhou  9 , Aaqil Khan  1 , Xu-Tong Lu  1   2 , Peng Deng  1   2 , Hang Zhou  1   2 , You-Wei Du  1   2
Affiliations

Regulatory effects of Hemin on prevention and rescue of salt stress in rapeseed (Brassica napus L.) seedlings

Hui-Min Zhao et al. BMC Plant Biol. .

Abstract

Background: Salt stress severely restricts rapeseed growth and productivity. Hemin can effectively alleviate salt stress in plants. However, the regulatory effect of Hemin on rapeseed in salt stress is unclear. Here, we analyzed the response and remediation mechanism of Hemin application to rapeseed before and after 0.6% (m salt: m soil) NaCl stress. Experiment using two Brassica napus (AACC, 2n = 38) rapeseed varieties Huayouza 158R (moderately salt-tolerant) and Huayouza 62 (strongly salt-tolerant). To explore the best optional ways to improve salt stress resistance in rapeseed.

Results: Our findings revealed that exogenous application of Hemin enhanced morph-physiological traits of rapeseed and significantly attenuate the inhibition of NaCl stress. Compared to Hemin (SH) treatment, Hemin (HS) significantly improved seedlings root length, seedlings height, stem diameter and accumulated more dry matter biomass under NaCl stress. Moreover, Hemin (HS) significantly improved photosynthetic efficiency, activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and decreased electrolyte leakage (EL) and malondialdehyde (MDA) content, thus resulting in the alleviation of oxidative membrane damage. Hemin (HS) showed better performance than Hemin (SH) under NaCl stress.

Conclusion: Hemin could effectively mitigate the adverse impacts of salt stress by regulating the morph-physiological, photosynthetic and antioxidants traits of rapeseed. This study may provide a basis for Hemin to regulate cultivated rapeseed salt tolerance and explore a better way to alleviate salt stress.

Keywords: Antioxidant system; Hemin; Photosynthesis; Rapeseed; Salinity stress.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Effect of Hemin prophylaxis and treatment on plant morphology of rapeseed under NaCl stress. CK: Control; H: Hemin; S: NaCl treatment; SH: NaCl + Hemin; HS: Hemin + NaCl
Fig. 2
Fig. 2
Effect of Hemin prophylaxis and treatment on fresh weight of rapeseed above (A, B) and below ground (C, D) under NaCl stress. Mean ± SE of three replicates. CK: Control; H: Hemin; S: salt treatment; SH: NaCl + Hemin; HS: Hemin + NaCl. Different letters indicate significant differences (p < 0.05)
Fig. 3
Fig. 3
Effect of Hemin prophylaxis and treatment on the dry weight of rapeseed upper (A, B) and lower ground (C, D) of rapeseed under NaCl stress. CK: Control; H: Hemin; S: NaCl treatment; SH: NaCl + Hemin; HS: Hemin + NaCl. Mean ± SE of three replicates. Different letters indicate significant differences (p < 0.05)
Fig. 4
Fig. 4
Effect of Hemin prophylaxis and treatment on electrolyte leakage rate (EL) (A, B) and malondialdehyde (MDA) (C, D) in rapeseed leaves under NaCl stress. CK: Control; H: Hemin; S: NaCl treatment; SH: NaCl + Hemin; HS: Hemin + NaCl. Mean ± SE of three replicates. Different letters indicate significant differences (p < 0.05)
Fig. 5
Fig. 5
Effect of Hemin prophylaxis and treatment on rapeseed’s relative chlorophyll content (SPAD) (A, B) under NaCl stress. CK: Control; H: Hemin; S: NaCl treatment; SH: NaCl + Hemin; HS: Hemin + NaCl. Mean ± SE of three replicates. Different letters indicate significant differences (p < 0.05)
Fig. 6
Fig. 6
Effect of Hemin prophylaxis and treatment on net photosynthetic rate (Pn) (A, B), stomatal conductance (Gs) (C, D), inter-cellular CO2 concentration (Ci) (E, F), and transpiration rate (Tr) (G, H) in rapeseed under NaCl stress. CK: Control; H: Hemin; S: NaCl treatment; SH: NaCl + Hemin; HS: Hemin + NaCl. Mean ± SE of three replicates. Different letters indicate significant differences (p < 0.05)
Fig. 7
Fig. 7
Effect of Hemin prophylaxis and treatment on superoxide dismutase (SOD) (A, B), peroxidase (POD) (C, D), ascorbate peroxidase (APX) (E, F), catalase (CAT) (G, H) in rapeseed under NaCl stress. CK: Control; H: Hemin; S: NaCl; SH: NaCl + Hemin; HS: Hemin + NaCl. Mean ± SE of three replicates. Different letters indicate significant differences (p < 0.05)
Fig. 8
Fig. 8
Effect of Hemin prophylaxis and treatment on soluble proteins (A, B) in rapeseed under NaCl stress. CK: Control; H: Hemin; S: NaCl; SH: NaCl + Hemin; HS: Hemin + NaCl. Mean ± SE of three replicates. Different letters indicate significant differences (p < 0.05)
See this image and copyright information in PMC

References

    1. Rossi L, Zhang W, Lombardini L, Ma X. The impact of cerium oxide nanoparticles on the salt stress responses of Brassica napus L. Environ Pollut. 2016;219:28–36. doi: 10.1016/j.envpol.2016.09.060. - DOI - PubMed
    1. Cui J, Yao D, Ma J, Ye X, Peng Y, Song J, et al. Nutrient uptake, physiological responses and growth of Tobacco (Nicotiana tabacum L.) in soil under composite salt stress. Pedosphere. 2022;32(6):893–904. doi: 10.1016/j.pedsph.2022.06.024. - DOI
    1. Ragaey MM, Sadak MS, Dawood MFA, Mousa NHS, Hanafy RS, Latef AA. Role of Signaling molecules Sodium Nitroprusside and Arginine in Alleviating Salt-Induced oxidative stress in wheat. Plants. 2022 doi: 10.3390/plants11141786. - DOI - PMC - PubMed
    1. Dawood MFA, Sofy MR, Mohamed HI, Sofy AR, Abdel-kader HAA. Hydrogen Sulfide modulates salinity stress in common Bean plants by maintaining osmolytes and regulating nitric oxide levels and antioxidant enzyme expression. J Soil Sci Plant Nutr. 2022;22(3):3708–26. doi: 10.1007/s42729-022-00921-w. - DOI
    1. Zhang X, Zhang L, Ma C, Su M, Wang J, Zheng S, et al. Exogenous strigolactones alleviate the photosynthetic inhibition and oxidative damage of cucumber seedlings under salt stress. Sci Hort. 2022;297:110962. doi: 10.1016/j.scienta.2022.110962. - DOI

LinkOut - more resources