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. 2023 Nov 3;28(21):7421.
doi: 10.3390/molecules28217421.

One Pot Synthesis of Nanofiber-Coated Magnetic Composites as Magnetic Dispersive Solid-Phase Extraction Adsorbents for Rapid Determination of Tetracyclines in Aquatic Food Products

Peipei Li  1 , Junlu Bai  2 , Pengfei He  1 , Junjie Zeng  1
Affiliations

One Pot Synthesis of Nanofiber-Coated Magnetic Composites as Magnetic Dispersive Solid-Phase Extraction Adsorbents for Rapid Determination of Tetracyclines in Aquatic Food Products

Peipei Li et al. Molecules. .

Abstract

A magnetic adsorbent based on a C-nanofiber (Fe3O4@C-NFs) nanocomposite was synthesized using a simple one-pot co-precipitation method. The characterized results showed that the obtained C-nanofiber-coated magnetic nanoparticles had many attractive features such as a large specific surface area and a highly interwoven and branched mesoporous structure, as well as distinguished magnetism. The nanocomposite was then used as an adsorbent in the magnetic solid phase extraction (MSPE) of four typical tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in aquatic products. The TCs in the extract were determined using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Experimental variables of MSPE, including the sorbent amount, pH condition, adsorption and desorption time, and desorption solvent, were investigated and optimized systematically. The method validation indicated that the developed method showed good linearity (R2 > 0.995) in the range of 1.0-200 ng/mL. The average recoveries at the spiked levels ranged from 90.7% to 102.7% with intra-day and inter-day relative standard deviations (RSDs, n = 6) ranging from 3.72% to 8.17% and 4.20% to 9.69%, respectively. The limit of detection (LOD) and limit of quantification (LOQ) for the four kinds of TCs were 0.7 μg/kg and 2.0 μg/kg, respectively. Finally, MSPE based on C-nanofiber-coated magnetic nanoparticles was successfully applied to TC analysis in real aquatic products (grass carp, large yellow croaker, snakehead, mandarin fish, Penaeus vannamei, swimming crab, etc.). Compared with traditional extraction methods, the proposed method for TC analysis in aquatic products is more sensitive, effective, recyclable, and environmentally friendly.

Keywords: C-nanofiber-coated magnetic composites; aquatic products; magnetic dispersive solid-phase extraction; mass spectrometry; tetracyclines.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SEM images of Fe3O4 (a,d), C–NF (b,e), and Fe3O4@C–NF nanocomposites (c,f).
Figure 2
Figure 2
(a) FT–IR spectra of Fe3O4 and Fe3O4@C–NFs; (b) XRD spectrum of Fe3O4@C–NFs; and (c) nitrogen adsorption/desorption isotherms of Fe3O4@C–NFs.
Figure 3
Figure 3
Effect of pH (a), adsorbent (b), adsorption time (c), eluent type (d), eluent volume (e), and desorption time (f) on the recovery response of the Fe3O4@C–NF nanoparticles in MSPE application to the 4 kinds of TCs.
Figure 4
Figure 4
MRM chromatogram (MS/MS) of the 4 kinds of TCs at a concentration of 5.0 μg kg−1 ((a), a standard solution of 2.0 ng/mL; (b), a blank fish sample; and (c), a spiked fish sample containing TCs at a concentration of 2.0 ng/mL).
Figure 5
Figure 5
A schematic diagram for the preparation of the Fe3O4@C–NF nanocomposites (a) and their application for TCs extraction (b).
See this image and copyright information in PMC

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