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Review
. 2024 Mar 1;34(2):44-51.
doi: 10.1097/MOU.0000000000001149. Epub 2023 Nov 14.

Restoring the barrier of chronically damaged urothelium using chondroitin sulfate glycosaminoglycan-replenishment therapy

Boy B Rozenberg  1 , Charlotte J van Ginkel, Dick A W Janssen
Affiliations
Review

Restoring the barrier of chronically damaged urothelium using chondroitin sulfate glycosaminoglycan-replenishment therapy

Boy B Rozenberg et al. Curr Opin Urol. .

Abstract

Purpose of review: This study aims to further understand the physiological mechanism of chondroitin sulfate treatment on the urinary bladder in cases of inflammation, by investigating the effect of chondroitin sulfate therapy on recovery of urothelial barrier in an in-vitro chronic injury model.

Recent findings: With inflammatory bladder conditions, the urothelial barrier seems decreased. Glycosaminoglycan (GAG) replacement therapy is supposed to help restore this barrier. Clinical studies on inflammatory bladder conditions are complicated because of the heterogeneous patient population, hence the need for preclinical models.

Summary: In a model using porcine urothelial cells, functional barrier (TEER) and barrier markers were assessed. Chronic urothelial damage was simulated through protamine sulfate instillations with and without subsequent chondroitin sulfate instillations during 3 days. Chondroitin sulfate instillations significantly improved TEER compared to protamine sulfate treatment only (TEER difference 310 Ω.cm 2 , P < 0.001). This consistent effect over 3 days resulted in a significant higher mean TEER value in the chondroitin sulfate treated group (difference 1855 Ω.cm 2 , P < 0.001). Enhanced recovery of chondroitin sulfate and other barrier markers was observed.Chondroitin sulfate therapy shows promise in facilitating the recovery of the urothelial barrier in cases of chronic damage. This preclinical study lends support to the use of clinical GAG replenishment therapy for patients with a chronically impaired urothelium.

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References

    1. Rudick CN, Bryce PJ, Guichelaar LA, et al. Mast cell-derived histamine mediates cystitis pain. PLoS One 2008; 3:e2096.
    1. Lewis SA. Everything you wanted to know about bladder epithelium but were afraid to ask. Am J Physiol Renal Physiol 2000; 278:F867–F874.
    1. Hauser PJ, Dozmorov MG, Bane BL, et al. Abnormal expression of differentiation related proteins and proteoglycan core proteins in the urothelium of patients with interstitial cystitis. J Urol 2008; 179:764–769.
    1. Hurst RE, Moldwin RM, Mulholland SG. Bladder defense molecules, urothelial differentiation, urinary biomarkers, and interstitial cystitis. Urology 2007; 69: (4 Suppl): 17–23.
    1. Hurst RE, Rhodes SW, Adamson PB, et al. Functional and structural characteristics of the glycosaminoglycans of the bladder luminal surface. J Urol 1987; 138:433–437.