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. 2023 Dec 12;11(6):e0183623.
doi: 10.1128/spectrum.01836-23. Epub 2023 Nov 14.

Development of a touchdown droplet digital PCR assay for the detection and quantitation of human papillomavirus 16 and 18 from self-collected anal samples

Gholamreza Haqshenas  1   2   3 , Suzanne M Garland  1   2   3 , Prisha Balgovind  2   3 , Alyssa Cornall  1   2   3 , Jennifer Danielewski  2   3 , Monica Molano  2 , Dorothy A Machalek  2   3   4 , Gerald Murray  1   2   3
Affiliations

Development of a touchdown droplet digital PCR assay for the detection and quantitation of human papillomavirus 16 and 18 from self-collected anal samples

Gholamreza Haqshenas et al. Microbiol Spectr. .

Abstract

The quantity of the human papillomavirus (HPV) is associated with disease outcome. We designed an accurate and precise digital PCR assay for quantitating HPV in anal samples, a sample type that is typically problematic due to the presence of PCR inhibitors.

Keywords: anal samples; droplet digital PCR; human papillomavirus.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
Quantitation of HPV16 and HPV18 using touchdown droplet digital PCR. Three broad-spectrum HPV primers and genotype-specific FAM-labeled probes (see Materials and Methods) were used to detect and quantify HPV16 and HPV18 from SiHa and HeLa cells, respectively. Negative and positive droplets are depicted as gray and colored, respectively. No probe cross-reactivity was observed (B).
Fig 2
Fig 2
Quantitation of HPV16 (n = 29) and HPV18 (n = 33) from Anyplex28 positive anal samples using TD-ddPCR. The horizontal lines of the box-and-whisker plots represent the median, and the whiskers represent the minimum and maximum values.
See this image and copyright information in PMC

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