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. 2023 Nov;43(6):1170-1182.
doi: 10.5851/kosfa.2023.e47. Epub 2023 Nov 1.

The Effect of a Chitosan/TiO2-Nanoparticle/ Rosmarinic Acid-Based Nanocomposite Coating on the Preservation of Refrigerated Rainbow Trout Fillets (Oncorhynchus mykiss)

Pınar Kizilkaya  1 , Mükerrem Kaya  2
Affiliations

The Effect of a Chitosan/TiO2-Nanoparticle/ Rosmarinic Acid-Based Nanocomposite Coating on the Preservation of Refrigerated Rainbow Trout Fillets (Oncorhynchus mykiss)

Pınar Kizilkaya et al. Food Sci Anim Resour. 2023 Nov.

Abstract

The aim of this study was to determine the effect of chitosan (CH)-based nanocomposite coating applications [chitosan+TiO2 (CHT) and chitosan+TiO2+rosmarinic acid (CHTRA)] on changes in quality attributes of rainbow trout fillets during cold storage (4°C). Fish fillets were randomly divided into four groups and subjected to treatments (CH, CHT, CHTRA, and control). After treatments, the groups were packaged under a modified atmosphere (40% CO2+30% O2+30% N2) and stored at 4°C for 18 days. During cold storage, the samples were subjected to physico-chemical and microbiological analyses. During storage, CH, CHT, and CHTRA treatments showed lower aerobic mesophilic and psychrotrophic bacteria counts than the control. However, the differences between coating treatments were not significant. The highest mean pH value was determined in the control group. As the storage time increased, the thiobarbituric acid reactive substances value increased. At the end of the storage period, no significant differences were observed between the treatments, including in the control group. The total volatile basic nitrogen (TVB-N) level in the control group was above 25 mg/100 g on day 15 of storage. However, the TVB-N level in the treatment groups was below 20 mg/100 g on day 18. It was also determined that coating application×storage period interaction had a significant effect on all color parameters (p<0.01). At the end of storage, the highest CIE L* was observed in CHTRA treatment. However, the value of this treatment did not differ from that of the CH treatment.

Keywords: TiO2; chitosan; nanocomposite coating; rosmarinic acid; trout fillet.

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Conflict of interest statement

The authors declare no potential conflicts of interest.

Figures

Fig. 1.
Fig. 1.. Effect of interaction of coating application×storage period on total aerobic mesophilic bacteria (A) and psychrotrophic bacteria (B) of rainbow trout fillets during cold storage.
A–F Different capital letters significant differences between storage periods for coating application. a,b Different small letters indicate significant differences between coating applications for the storage periods. C, uncoated; CH, chitosan; CHT, chitosan+TiO2; CHTRA, chitosan+TiO2+rosmarinic acid.
Fig. 2.
Fig. 2.. Effect of interaction of coating application×storage period on pH value of rainbow trout fillets during cold storage.
A,B Different capital letters significant differences between storage period for coating application. a–c Different small letters indicate significant differences between coating applications for the storage periods. C, uncoated; CH, chitosan; CHT, chitosan+TiO2; CHTRA, chitosan+TiO2+ rosmarinic acid.
Fig. 3.
Fig. 3.. Effect of the interaction of coating application×storage period on thiobarbituric acid reactive substances (TBARS) value of rainbow trout fillets during cold storage.
A–F Different capital letters significant differences between storage period for coating application. a–c Different small letters indicate significant differences between coating applications for the storage periods. MDA, malondialdehyde; C, uncoated; CH, chitosan; CHT, chitosan+TiO2; CHTRA, chitosan+TiO2+rosmarinic acid.
Fig. 4.
Fig. 4.. Effect of the interaction of coating application×storage period on total volatile basic nitrogen (TVB-N) level of rainbow trout fillets during cold storage.
A–D Different capital letters significant differences between storage period for coating application. a,b Different small letters indicate significant differences between coating applications for the storage periods. C, uncoated; CH, chitosan; CHT, chitosan+ TiO2; CHTRA, chitosan+TiO2+rosmarinic acid.
Fig. 5.
Fig. 5.. Effect of the interaction of coating application×storage period on CIE L* of rainbow trout fillets during cold storage.
A–D Different capital letters significant differences between storage period for coating application. a–c Different small letters indicate significant differences between coating applications for the storage periods. C, uncoated; CH, chitosan; CHT, chitosan+TiO2; CHTRA, chitosan+TiO2+rosmarinic acid.
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