Artificial Cultivation Characteristics and Bioactive Effects of Novel Tropicoporus linteus (Syn. Phellinus linteus) Strains HN00K9 and HN6036 in Korea

Abstract

Phellinus strains were collected from different areas in Korea. Of them, the fast mycelial growing strains were artificially cultivated on the oak logs to produce fruiting body. The varieties, Phellinus linteus ASI26099 (Korea Sanghwang) and P. baumii PBJS (Jangsoo Sanghwang) were grown under the same conditions as controls. Their cultivating characteristics including mycelial colonization, pinhead formation, and fruiting body formation rate were investigated on the logs. Basidiocarps of Phellinus strains HN00K9, HN6036, and ASI26099 were concentrically zonate and shallowly sulcate, and dark chestnut showing typical characteristics of Tropicoporus linteus (synonyum: P. linteus, Inonotus linteus, polyporus linteus), which is distinguishably different to PBJS. HN00K9 showed the highest yield of fruiting body among the mushroom strains. The β-glucan content in fruiting bodies of HN00K9 was 20% higher than those of other strains. Bioactive effects of polysaccharide samples from fruiting bodies of Phellinus strains, HN00K9, HN6036, ASI26099, and PBJS were assessed on cell viability and cytokine (IL-6 and TNF-α) inhibition and finally on anticancer to different human cancer cells.

Keywords: Artificial cultivation; Tropicoporus linteus; bioactivity; fruiting bodies; morphological characteristics.

Figure 1.

Morphological characteristics of mycelia of Phellinus strains grown on yeast malt glucose (YGM) media for 15 days at 25 °C.

Figure 2.

Formation of pinheads and fruiting bodies of Phellinus strains HN00K9 and PBJS on oak logs in a mushroom house.

Figure 3.

Morphological characteristics of fruiting bodies produced from Phellinus strains HN00K9, ASI26099, HN6036, and PBJS formed on the oak logs.

Figure 4.

Scanning electron microscopic features of (A) lateral sections and (B) pore surface of basidiocarps derived from Phellinus strains HN00K9, ASI26099, HN6036, and PBJS.

Figure 5.

Viability of RAW 264.7 cells in polysaccharide from fruiting bodies of Inonotus linteus complex strains HN00K9, ASI 26099, HN6036, and PBJS. The cells were treated with different concentrations of polysaccharides from fruiting bodies for 24 h. LPS activated cell viability was determined by MTT assay. The values are the mean ± SD from three independent experiments. Significant differences between the polysaccharide-treated and untreated groups were analyzed using Student’s t-test (*p < 0.05, **p < 0.005, ***p < 0.0005).

Figure 6.

Effect of polysaccharides from fruiting body of Inonotus linteus complex strains on the production of TNF-α and IL-6 in RAW 264.7 cells. RAW264.7 cells were treated with different concentrations (125, 250, 500, 1000 ㎍/mL) of polysaccharides extracted from fruiting bodies of Phellinus strains, HN00K9, ASI 26099, HN6036 and PBJS for 24 h under treatment of LPS (100 ng/mL). Each value is expressed as mean ± SD in triplicate experiments. Significant differences between the polysaccharide-treated and untreated groups were analyzed using Student’s t-test (*p < 0.05, **p < 0.005, ***p < 0.0005).