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Randomized Controlled Trial
. 2023 Nov 13;69(12):e20230961.
doi: 10.1590/1806-9282.20230961. eCollection 2023.

Cytogenetic changes in oral mucosa cells from individuals submitted to oral human immunodeficiency virus pre-exposure prophylaxis use

Maria Esther Suarez Alpire  1 , Daniel Vitor de Souza  1 , Carolina Marquez da Costa Brites Masutti  2 , Marcos Montani Caseiro  3 , Daniel Araki Ribeiro  1
Affiliations
Randomized Controlled Trial

Cytogenetic changes in oral mucosa cells from individuals submitted to oral human immunodeficiency virus pre-exposure prophylaxis use

Maria Esther Suarez Alpire et al. Rev Assoc Med Bras (1992). .

Abstract

Objective: The objective of this study was to evaluate cytogenetic changes in individuals submitted to oral human immunodeficiency virus pre-exposure prophylaxis use through the micronucleus test in oral mucosa.

Methods: This study consisted of 37 individuals, of whom 17 comprised the pre-exposure prophylaxis group and 20 comprised the control group. A total of 2,000 cells per slide were analyzed for the determination of micronuclei, binucleation, nuclear buds, and cytotoxicity parameters: pyknosis, karyolysis, and karyorrhexis (KR), in a double-blind manner. The repair index was also evaluated in this setting.

Results: In the mutagenicity parameters, the pre-exposure prophylaxis group showed increased frequencies of micronuclei (p=0.0001), binucleation (p=0.001), and nuclear buds (p=0.07). Regarding the cytotoxicity parameters, there was an increase with a statistical difference (p≤0.05) in the karyorrhexis frequency (p=0.001). Additionally, the repair system efficiency decreased in the pre-exposure prophylaxis group.

Conclusion: These results indicate that individuals undergoing pre-exposure prophylaxis use have geno- and cytotoxicity in oral mucosal cells.

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Conflict of interest statement

Conflicts of interest: the authors declare there is no conflicts of interest.

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