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. 2024 Aug;196(8):4807-4822.
doi: 10.1007/s12010-023-04755-9. Epub 2023 Nov 16.

Ginkgo biloba Extract 50 (GBE50) Exerts Antifibrotic and Antioxidant Effects on Pulmonary Fibrosis in Mice by Regulating Nrf2 and TGF-β1/Smad Pathways

Wei Liang #  1 , Hongmei Yang #  1   2 , Ling Pan  3 , Sizun Wei  4 , Zhanhua Li  1 , Pengfei Zhang  5 , Ruixiang Li  6 , Yangcong Wu  7 , Maohua Liu  7 , Xiaohong Liu  8
Affiliations

Ginkgo biloba Extract 50 (GBE50) Exerts Antifibrotic and Antioxidant Effects on Pulmonary Fibrosis in Mice by Regulating Nrf2 and TGF-β1/Smad Pathways

Wei Liang et al. Appl Biochem Biotechnol. 2024 Aug.

Erratum in

Abstract

Background: Pulmonary fibrosis (PF) is a progressive lung disorder with a poor prognosis. GBE50 is a new standardized Ginkgo biloba extract that has been widely used in cardiovascular diseases. However, the protective mechanism of GBE50 against PF remains to be elucidated.

Methods: C57BL/6J mice were treated with bleomycin (Bleo) to induce PF in the presence or absence of GBE50. Protein content in bronchoalveolar lavage fluid (BALF) and wet weight/dry weight ratio were examined for analysis of pulmonary edema. Hematoxylin-eosin staining and Masson trichrome staining were used for histopathological observation of murine lung tissues. Ashcroft score was used for semi-quantitation of lung fibrosis degree. RT-qPCR was utilized for assessing mRNA levels of pro-fibrotic mediators in lung tissues. TUNEL staining was implemented for cell apoptosis assessment. The levels of oxidative stress- and inflammation-related markers were evaluated by corresponding commercial assay kits. Western blotting was used to evaluate levels of nuclear factor erythroid 2-related factor 2 (Nrf2) signaling- and transforming growth factor (TGF)-β1/SMAD signaling-related proteins.

Results: GBE50 alleviated lung injury and severity of fibrosis, reduced collagen deposition and cell apoptosis in lung tissues, and suppressed inflammatory response and oxidative stress injury in Bleo-stimulated PF mice. GBE50 activated Nrf2 signaling pathway and inactivated TGF-β1/SMAD signaling pathway in the lungs of Bleo-induced PF mice. Inhibition of Nrf2 signaling reversed GBE50-mediated inactivation of TGF-β1/SMAD signaling and attenuation of inflammation and oxidative stress in Bleo-induced PF mice.

Conclusion: GBE50 protects against Bleo-induced PF in mice by mitigating fibrosis, inflammation and oxidative stress via Nrf2 and TGF-β1/SMAD signaling pathways.

Keywords: Ginkgo biloba Extract 50; Bleomycin; Inflammation; Oxidative Stress; Pulmonary Fibrosis.

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References

    1. Saleh, M., Fotook Kiaei, S. Z., & Kavianpour, M. (2022). Application of Wharton jelly-derived mesenchymal stem cells in patients with pulmonary fibrosis. Stem Cell Research & Therapy, 13(1), 71. - DOI
    1. Chen, I. C. (2022). Evaluation of Proteasome inhibitors in the treatment of Idiopathic Pulmonary Fibrosis. Cells, 11(9).
    1. Herrera, J., Henke, C. A., & BitTerman, P. B. (2018). Extracellular matrix as a driver of Progressive fibrosis. J Clin Invest, 128(1), 45–53. - DOI - PubMed - PMC
    1. Martinez, F. J., et al. (2017). Idiopathic Pulmonary Fibrosis. Nat Rev Dis Primers, 3, 17074. - DOI - PubMed
    1. Raghu, G., et al. (2011). An official ATS/ERS/JRS/ALAT statement: Idiopathic Pulmonary Fibrosis: Evidence-based guidelines for diagnosis and management. American Journal of Respiratory and Critical Care Medicine, 183(6), 788–824. - DOI - PubMed - PMC

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