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. 1979 Jun 11;6(7):2483-97.
doi: 10.1093/nar/6.7.2483.

The use of R-looping for structural gene identification and mRNA purification

J L Woolford Jr, M Rosbash
Free PMC article

The use of R-looping for structural gene identification and mRNA purification

J L Woolford Jr et al. Nucleic Acids Res. .
Free PMC article

Abstract

A method is presented for the purification of mRNAs and the identification of structural gene sequences in recombinant DNA molecules. RNA is hybridized to double-stranded linear DNA such that R-loops are formed between most DNAs and their complementary RNA sequences. These R-loops are purified from unhybridized RNAs by gel filtration chromatography in the presence of a high concentration of salt. The complementary RNAs are released from the R-loops by heating, and are assayed by gel electrophoresis or cell free translation to determine their purity and to identify the proteins for which they code. We have demonstrated that recombinant DNAs containing sequences for abundant or moderately abundant mRNAs of Saccharomyces cerevisiae can be identified by this means.

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References

    1. J Mol Biol. 1965 Feb;11:373-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1217-21 - PubMed
    1. Nucleic Acids Res. 1977;4(5):1539-52 - PubMed
    1. J Mol Biol. 1977 Feb 5;109(4):559-87 - PubMed
    1. Cell. 1977 Feb;10(2):177-92 - PubMed

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