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. 1979 Jun 11;6(7):2583-99.
doi: 10.1093/nar/6.7.2583.

Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction

H S Sista, R T Loder, M H Caruthers
Free PMC article

Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction

H S Sista et al. Nucleic Acids Res. .
Free PMC article

Abstract

Chemical and enzymatic methods were used to synthesize a transition (AT to GC) and a transversion (AT to TA) at a lac operator site known to interact with lac repressor through the thymine 5 methyl group. These operators also contained a poly(dA) . poly(dT) tail 8 to 12 base pairs in length at one end. Results suggest that the steric constraints of lac repressor relative to the position of the 5 methyl group are quite critical. For example a seven fold reduction in stability was observed for the transversion. Results also suggest that the operator spans at least 21 base pairs.

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References

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    1. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3292-6 - PubMed
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