In vitro studies on the effect of yolk sac antisera on functions of the visceral yolk sac: I. Pinocytosis and transport of small molecules
- PMID: 3798369
- DOI: 10.1002/tera.1420340313
In vitro studies on the effect of yolk sac antisera on functions of the visceral yolk sac: I. Pinocytosis and transport of small molecules
Abstract
The production of congenital malformations by the administration of teratogenic antisera to pregnant animals has been reported from many laboratories. This work has focused our attention on the importance of the yolk sac placenta in supporting the rat embryo during early organogenesis and the significance of yolk sac dysfunction in rodent teratogenesis. The studies reported in this article deal with the effect of teratogenic antisera on the process of yolk sac transport; specifically pinocytosis (as measured by 14C-sucrose uptake) and small-molecule transport utilizing 14C-alpha-aminoisobutyric acid (AIB) and 3H-2-deoxyglucose (DOG). We sought to determine whether several different yolk sac localizing antibodies interfere with these transport processes, and, if so, which transport processes were most affected. The results of the experiments indicated that teratogenic antisera interfered with the process of pinocytosis in the yolk sac and that pinocytosis can be reduced as much as 40%. Nonteratogenic antisera, even when they localized in the yolk sac, did not interfere with the process of pinocytosis. Furthermore, the teratogenic antisera did not interfere with the transport of small molecules (either AIB or DOG) in the yolk sac. These results indicated that while fluorescent localization of an antiserum in the yolk sac did not invariably indicate the potential for teratogenicity, it is likely that the reduction in pinocytosis may directly correlate with the teratologic and embryopathic events. This work reaffirms the view that the yolk sac in important during rodent organogenesis and that yolk sac dysfunction can play an important role in the development of congenital malformations.(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
-
Ultrastructure and function of the rat yolk sac: damage caused by teratogenic anti-VYS serum and recovery.Teratology. 1991 Aug;44(2):181-92. doi: 10.1002/tera.1420440206. Teratology. 1991. PMID: 1925977
-
Experimental yolk sac dysfunction as a model for studying nutritional disturbances in the embryo during early organogenesis.Teratology. 1990 Apr;41(4):405-13. doi: 10.1002/tera.1420410406. Teratology. 1990. PMID: 2187260 Review.
-
Multiple antigens in the rat visceral yolk sac induce teratogenic antisera.Teratology. 1991 Jun;43(6):601-8. doi: 10.1002/tera.1420430615. Teratology. 1991. PMID: 1882351
-
[Inhibition on the endocytosis of early visceral yolk sac induced by homocysteine in rats].Wei Sheng Yan Jiu. 2002 Feb;31(1):32-3. Wei Sheng Yan Jiu. 2002. PMID: 12561569 Chinese.
-
Cell physiology of the rat visceral yolk sac: a study of pinocytosis and lysosome function.Teratology. 1990 Apr;41(4):383-93. doi: 10.1002/tera.1420410404. Teratology. 1990. PMID: 2187258 Review.
Cited by
-
Maternofetal transport of vitamin B12: role of TCblR/CD320 and megalin.FASEB J. 2017 Jul;31(7):3098-3106. doi: 10.1096/fj.201700025R. Epub 2017 Mar 28. FASEB J. 2017. PMID: 28351841 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources