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. 2024 Feb;65(1):113-120.
doi: 10.1007/s13353-023-00810-7. Epub 2023 Nov 22.

NADPH oxidase 4-mediating oxidative stress contributes to endometriosis

Xiaojie Wang #  1 , Xiaona Jiang #  1 , Xin Lv #  1 , Xinshu Wang  1 , Aimin Lin  1 , Yangyang Li  2
Affiliations

NADPH oxidase 4-mediating oxidative stress contributes to endometriosis

Xiaojie Wang et al. J Appl Genet. 2024 Feb.

Abstract

NADPH oxidase 4 (NOX4) plays an important role in the regulation of oxidative stress, which is associated with endometriosis. This study aims to investigate the effects of NOX4 in endometriosis and its molecular mechanisms. Clinical specimens were collected, and human endometrial stromal cells (HESCs) were isolated. The knockdown of NOX4 cell lines was established on the HESCs and induced by peritoneal fluid. The levels of NOX4 were determined by using immunohistochemistry (IHC) staining, western blotting, and qPCR, respectively. The levels of oxidative stress markers were determined by using western blotting and ELISAs, respectively. The correlation of NOX4 and oxidative stress markers was analyzed by the Pearson correlation coefficient. The levels of NOX4 were dramatically elevated in the ectopic endometrium. Besides, oxidative stress biomarkers were also dysregulated in the ectopic endometrium as compared to the normal endometrium. Pearson's correlation coefficient analysis revealed a relationship between NOX4 and oxidative stress biomarkers in the ectopic endometrium. NOX4 modulated the expressions of oxidative stress markers in endometrial stromal cells stimulated by the peritoneal fluid from endometriosis. The effects of NOX4 on endometriosis are in part by its regulatory effects against oxidative stress.

Keywords: Catalase; Endometrial stromal cells; Endometriosis; NADPH oxidase 4; Oxidative stress.

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References

    1. Amreen S, Kumar P, Gupta P, Rao P (2019) Evaluation of oxidative stress and severity of endometriosis. J Hum Reprod Sci 12(1):40 - DOI - PubMed - PMC
    1. Basuroy S, Bhattacharya S, Leffler CW, Parfenova H (2009) Nox4 NADPH oxidase mediates oxidative stress and apoptosis caused by TNF-α in cerebral vascular endothelial cells. Am J Physiol Cell Physiol 296(3):C422–C432 - DOI - PubMed
    1. Chen F, Haigh S, Barman S, Fulton DJ (2012) From form to function: the role of Nox4 in the cardiovascular system. Front Physiol 3:412 - DOI - PubMed - PMC
    1. Cramer DW, Missmer SA (2002) The epidemiology of endometriosis. Ann N Y Acad Sci 955(1):11–22 - DOI - PubMed
    1. Degasper C, Brunner A, Sampson N, Tsibulak I, Wieser V, Welponer H, Marth C, Fiegl H, Zeimet AG (2019) NADPH oxidase 4 expression in the normal endometrium and in endometrial cancer. Tumor Biol 41(2):1010428319830002 - DOI

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