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. 2023 Nov 21;13(1):20389.
doi: 10.1038/s41598-023-47682-9.

Minimally invasive management of vital teeth requiring root canal therapy

E Karatas  1   2 , M Hadis  2 , W M Palin  2 , M R Milward  2 , S A Kuehne  2   3 , J Camilleri  4
Affiliations

Minimally invasive management of vital teeth requiring root canal therapy

E Karatas et al. Sci Rep. .

Abstract

The present study aimed to investigate the possible use of a non-instrumentation technique including blue light irradiation for root canal cleaning. Extracted human single rooted teeth were selected. Nine different groups included distilled water, NaOCl, intra-canal heated NaOCl, and NaOCl + EDTA irrigation after either instrumentation or non-instrumentation, and a laser application group following non-instrumentation technique. The chemical assessment of the root canal dentine was evaluated using energy dispersive spectroscopy (EDS) and Fourier transform infrared (FT-IR) spectroscopy. Surface microstructural analyses were performed by using scanning electron microscopy (SEM). The antimicrobial efficacy of different preparation techniques was evaluated using microbial tests. Light application didn't change the calcium/phosphorus, carbonate/phosphate and amide I/phosphate ratios of the root canal dentin. The root canal dentin preserved its original chemistry and microstructure after light application. The instrumentation decreased the carbonate/phosphate and amide I/phosphate ratios of the root canal dentin regardless of the irrigation solution or technique (p < 0.05). The application of light could not provide antibacterial efficacy to match the NaOCl irrigation. The NaOCl irrigation both in the non-instrumentation and instrumentation groups significantly reduced the number of bacteria (p < 0.05). The use of minimally invasive root canal preparation techniques where the root canal is not instrumented and is disinfected by light followed by obturation with a hydraulic cement sealer reduced the microbial load and preserved the dentin thus may be an attractive treatment option for management of vital teeth needing root canal therapy.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Light transmission levels (µW/cm2/nm) for (a) 2 and 4 mm samples and (b) 6, 10 and 22 mm samples. The absolute irradiance was calculated using the area under the curve.
Figure 2
Figure 2
The change in temperature recorded during the intracanal laser application with 200 µm fibre either in an empty dry root canal (a) or with the presence of 0.1 mL of distilled water (b).
Figure 3
Figure 3
The change in temperature recorded during the extra-canal laser application (from the root canal orifice) with 105 µm fibre in an empty dry root canal.
Figure 4
Figure 4
The changes in temperature recorded during intra-canal heat application with SuperEndo either in an empty dry root canal (a), with the presence of distilled water (b), or NaOCl (c).
Figure 5
Figure 5
SEM images of root canal surface at ×3 K magnification after different preparation procedure.
Figure 6
Figure 6
SEM images of horizontal root sections at ×1 K magnification after obturation with AH Plus following different preparation procedures. GP: gutta-percha, D: the dentin.
Figure 7
Figure 7
SEM images of horizontal root sections at ×1 K magnification after obturation with BioRoot RCS RCS following different preparation procedures. GP: gutta-percha, D: dentin.
Figure 8
Figure 8
Distribution of teeth according to the groups.
Figure 9
Figure 9
A schematic presentation of the light transmission test.
See this image and copyright information in PMC

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