Expression of the cloned S4 gene of reovirus serotype 3 in transformed eucaryotic cells: enrichment of the viral protein in the crude initiation factor fraction

Abstract

The sigma 3 protein of reovirus is believed to play a role in the control of protein synthesis in reovirus-infected cells. In this paper we describe the establishment of a line of L-cells expressing the sigma 3 protein from the cloned S4 gene of reovirus serotype 3, under the control of the SV40 early promoter. The protein was enriched in the crude initiation factor fraction prepared by a high-salt wash of ribosomes. There was no apparent detrimental effect on the cell line.