Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Nov 10;9(11):1096.
doi: 10.3390/jof9111096.

Four New Endophytic Apiospora Species Isolated from Three Dicranopteris Species in Guizhou, China

Jing-Yi Zhang  1   2   3 , Meng-Lan Chen  1 , Saranyaphat Boonmee  2   3 , Yu-Xuan Wang  1 , Yong-Zhong Lu  1
Affiliations

Four New Endophytic Apiospora Species Isolated from Three Dicranopteris Species in Guizhou, China

Jing-Yi Zhang et al. J Fungi (Basel). .

Abstract

Endophytic fungi isolated from medicinal ferns serve as significant natural resources for drug precursors or bioactive metabolites. During our survey on the diversity of endophytic fungi from Dicranopteris species (a genus of medicinal ferns) in Guizhou, Apoiospora was observed as a dominant fungal group. In this study, seven Apiospora strains, representing four new species, were obtained from the healthy plant tissues of three Dicranopteris species-D. ampla, D. linearis, and D. pedata. The four new species, namely Apiospora aseptata, A. dematiacea, A. dicranopteridis, and A. globosa, were described in detail with color photographs and subjected to phylogenetic analyses using combined LSU, ITS, TEF1-α, and TUB2 sequence data. This study also documented three new hosts for Apiospora species.

Keywords: asexual morph; dematiaceous conidia; medicinal ferns; phylogeny; seven taxa; taxonomy.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The phylogenetic tree generated from ML analysis is based on a concatenated LSU–ITS–TEF1α–TUB2 dataset for the family Apiosporaceae. Bootstrap support values for ML greater than 60% and Bayesian posterior probabilities (PPs) greater than 0.95 were indicated above or below the nodes as ML/PP. Seiridium phylicae (CPC 19962 and CPC 19965) were selected as the outgroup taxa. The newly generated sequences are shown in red.
Figure 1
Figure 1
The phylogenetic tree generated from ML analysis is based on a concatenated LSU–ITS–TEF1α–TUB2 dataset for the family Apiosporaceae. Bootstrap support values for ML greater than 60% and Bayesian posterior probabilities (PPs) greater than 0.95 were indicated above or below the nodes as ML/PP. Seiridium phylicae (CPC 19962 and CPC 19965) were selected as the outgroup taxa. The newly generated sequences are shown in red.
Figure 2
Figure 2
Apiospora aseptata (HKAS 129875, holotype). (a,b) Cultures on PDA from above and below; (c) colonies on PDA; (d–g) conidiogenous cells with conidia; (h–j) conidia. Scale bars: (d) 20 μm; (e–j) 10 μm.
Figure 3
Figure 3
Apiospora dematiacea (HKAS 129910, Holotype). (a,b) Cultures on PDA from above and below; (c) colonies on PDA; (d–i) conidiogenous cells with conidia; (j–p) conidia. Scale bars: (d) 50 μm; (e–l) 20 μm; (m–p) 10 μm.
Figure 4
Figure 4
Apiospora dicranopteridis (HKAS 129877, holotype). (a,b) Cultures on PDA from above and below; (c,d) colonies on PDA; (d–l) conidiophores, conidiogenous cells with conidia. Scale bars: (e,f) 50 μm; (g–l) 20 μm.
Figure 5
Figure 5
Apiospora globosa (HKAS 129921, holotype). (a,b) Cultures on WA from above and below; (c) colonies on WA; (d–f) conidiophores with conidia; (g–i) conidia. Scale bars: (d–f) 20 μm; (g–i) 10 μm.
See this image and copyright information in PMC

References

    1. Hyde K., Fröhlich J., Taylor J. Fungi from palms. XXXVI. Reflections on unitunicate ascomycetes with apiospores. Sydowia. 1998;50:21–80.
    1. Senanayake I.C., Maharachchikumbura S.S.N., Hyde K.D., Bhat J.D., Jones E.G., McKenzie E.H., Dai D.Q., Daranagama D.A., Dayarathne M.C., Goonasekara I.D. Towards unraveling relationships in Xylariomycetidae (Sordariomycetes) Fungal Divers. 2015;73:73–144. doi: 10.1007/s13225-015-0340-y. - DOI
    1. Hyde K.D., Norphanphoun C., Maharachchikumbura S., Bhat D., Jones E., Bundhun D., Chen Y., Bao D., Boonmee S., Calabon M. Refined families of Sordariomycetes. Mycosphere. 2020;11:305–1059. doi: 10.5943/mycosphere/11/1/7. - DOI
    1. Jiang N., Voglmayr H., Ma C.-Y., Xue H., Piao C.-G., Li Y. A new Arthrinium-like genus of Amphisphaeriales in China. MycoKeys. 2022;92:27. doi: 10.3897/mycokeys.92.86521. - DOI - PMC - PubMed
    1. Samarakoon M.C., Hyde K.D., Maharachchikumbura S.S., Stadler M., Gareth Jones E., Promputtha I., Suwannarach N., Camporesi E., Bulgakov T.S., Liu J.-K. Taxonomy, phylogeny, molecular dating and ancestral state reconstruction of Xylariomycetidae (Sordariomycetes) Fungal Divers. 2022;112:1–88. doi: 10.1007/s13225-021-00495-5. - DOI

LinkOut - more resources