Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Nov 6;8(7):528.
doi: 10.3390/biomimetics8070528.

Complex Evaluation of Nanocomposite-Based Hydroxyapatite for Biomedical Applications

Daniela Predoi  1 , Simona Liliana Iconaru  1 , Steluta Carmen Ciobanu  1 , Nicolas Buton  2 , Mihai Valentin Predoi  3
Affiliations

Complex Evaluation of Nanocomposite-Based Hydroxyapatite for Biomedical Applications

Daniela Predoi et al. Biomimetics (Basel). .

Abstract

A magnesium-doped hydroxyapatite in chitosan matrix (MgHApC) sample was developed as a potential platform for numerous applications in the pharmaceutical, medical, and food industries. Magnesium-doped hydroxyapatite suspensions in the chitosan matrix were obtained by the coprecipitation technique. The surface shape and morphological features were determined by scanning electron microscopy (SEM). The hydrodynamic diameter of the suspended particles was determined by Dynamic light scattering (DLS) measurements. The stability of MgHApC suspensions was evaluated by ultrasonic measurements. The hydrodynamic diameter of the MgHApC particles in suspension was 29.5 nm. The diameter of MgHApC particles calculated from SEM was 12.5 ± 2 nm. Following the SEM observations, it was seen that the MgHApC particles have a spherical shape. The Fourier-transform infrared spectroscopy (FTIR) studies conducted on MgHApC proved the presence of chitosan and hydroxyapatite in the studied specimens. In vitro antimicrobial assays were performed on Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 10231 microbial strains. The antimicrobial experiments showed that MgHApC exhibited very good antimicrobial properties against all the tested microorganisms. More than that, the results of the in vitro studies revealed that the antimicrobial properties of the samples depend on the incubation time. The evaluation of the sample's cytotoxicity was performed using the human colon cancer (HCT-8) cell line. Our results suggested the great potential of MgHApC to be used in future applications in the field of biomedical applications (e.g., dentistry, orthopedics, etc.).

Keywords: antimicrobial activity; chitosan; cytotoxicity; magnesium doped hydroxyapatite; stable suspensions.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of this study, in the collection, analysis, or interpretation of data, in the writing of this manuscript, or in the decision to publish this results.

Figures

Figure 1
Figure 1
The time evolution of the ultrasonic signal relative amplitudes through the MgHApC suspension.
Figure 2
Figure 2
Frequency spectra of the recorded signal for the MgHApC suspension and the reference liquid.
Figure 3
Figure 3
Attenuation of ultrasonic signals vs. frequency for MgHApC suspension.
Figure 4
Figure 4
Attenuation of ultrasonic signals vs. time for MgHApC suspension.
Figure 5
Figure 5
Intensity size distribution (a) and volume size distribution (b) of suspension MgHApC.
Figure 6
Figure 6
Low magnification SEM micrographs (a) and EDS spectrum (b) of MgHApC particles.
Figure 7
Figure 7
HRSEM micrographs (a) and 3D HRSEM micrographs of MgHApC particles (b). Size distribution of the MgHApC particles (c).
Figure 8
Figure 8
Elemental mapping cartographies were obtained for the MgHApC sample.
Figure 9
Figure 9
Three-dimensional representation of elemental mapping cartographies obtained for MgHApC.
Figure 10
Figure 10
Typical absorbance FTIR spectra of MgHApC, MgHAp, and HAp samples.
Figure 11
Figure 11
FTIR—second derivative spectra obtained for the MgHApC, MgHAp, and HAp samples.
Figure 12
Figure 12
FTIR—second derivative spectra obtained for the MgHApC sample in the 1400–1800 cm−1 spectral domain.
Figure 13
Figure 13
The graphical representation of the antimicrobial activity of MgHApC, MgHAp, HApC, and HAp suspensions against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 10231 microbial strains after 24, 48, and 72 h of incubation.
Figure 14
Figure 14
MTT assay of HCT-8 cells incubated with MgHApC suspensions for 24, 48, and 72 h. p ≤ 0.05 was accepted as statistically significant.
See this image and copyright information in PMC

References

    1. Fakhri E., Eslami H., Maroufi P., Pakdel F., Taghizadeh S., Ganbarov K., Yousefi M., Tanomandf A., Yousefi B., Mahmoudi S., et al. Chitosan biomaterials application in dentistry. Int. J. Biol. Macromol. 2020;162:956–974. doi: 10.1016/j.ijbiomac.2020.06.211. - DOI - PubMed
    1. Mehrabani M.G., Karimian R., Mehramouz B., Rahimi M., Kafil H.S. Preparation of biocompatible and biodegradable silk fibroin/chitin/silver nanoparticles 3D scaffolds as a bandage for antimicrobial wound dressing. Int. J. Biol. Macromol. 2018;114:961–971. doi: 10.1016/j.ijbiomac.2018.03.128. - DOI - PubMed
    1. Chan B., Leong K. Scaffolding in tissue engineering: General approaches and tissue specific considerations. Eur. Spine J. 2008;17:467–479. doi: 10.1007/s00586-008-0745-3. - DOI - PMC - PubMed
    1. Cross L.M., Thakur A., Jalili N.A., Detamore M., Gaharwar A.K. Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces. Acta Biomater. 2016;42:2–17. doi: 10.1016/j.actbio.2016.06.023. - DOI - PubMed
    1. Liang J., Peng X., Zhou X., Zou J., Cheng L. Emerging Applications of Drug Delivery Systems in Oral Infectious Diseases Prevention and Treatment. Molecules. 2020;25:516. doi: 10.3390/molecules25030516. - DOI - PMC - PubMed

LinkOut - more resources