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. 2024 Feb:393:130094.
doi: 10.1016/j.biortech.2023.130094. Epub 2023 Nov 22.

Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Silvanir  1 , Sin Yuan Lai  2 , Azren Aida Asmawi  3 , Kit Wayne Chew  4 , Cheng Loong Ngan  5
Affiliations

Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Silvanir et al. Bioresour Technol. 2024 Feb.

Abstract

Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.

Keywords: Aqueous two-phase system; Biorefinery; Chlorella vulgaris; Rotor-stator homogenization; Separation.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.