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. 2023 Nov 14;11(11):3053.
doi: 10.3390/biomedicines11113053.

The Analysis of Selected miRNAs and Target MDM2 Gene Expression in Oral Squamous Cell Carcinoma

Karolina Gołąbek  1 , Dorota Hudy  1 , Jadwiga Gaździcka  1 , Katarzyna Miśkiewicz-Orczyk  2 , Magdalena Nowak-Chmura  3 , Marek Asman  1 , Katarzyna Komosińska-Vassev  4 , Wojciech Ścierski  2 , Wojciech Golusiński  5 , Maciej Misiołek  2 , Joanna Katarzyna Strzelczyk  1
Affiliations

The Analysis of Selected miRNAs and Target MDM2 Gene Expression in Oral Squamous Cell Carcinoma

Karolina Gołąbek et al. Biomedicines. .

Abstract

MiRNAs could play an important role in tumorigenesis and progression. The oncoprotein MDM2 (murine double minute 2) was identified as a negative regulator of the tumour suppressor p53. This study aims to analyse the expression of the MDM2 target miRNA candidates (miR-3613-3p, miR-371b-5p and miR-3658) and the MDM2 gene in oral squamous cell carcinoma tumour and margin samples and their association with the selected socio-demographic and clinicopathological characteristics. The study group consisted of 50 patients. The miRNAs and MDM2 gene expression levels were assessed by qPCR. The expression analysis of the miRNAs showed the expression of only one of them, i.e., miR-3613-3p. We found no statistically significant differences in the miR-3613-3p expression in tumour samples compared to the margin samples. When analysing the effect of smoking on miR-3613-3p expression, we demonstrated a statistically significant difference between smokers and non-smokers. In addition, we showed an association between the miR-3613-3p expression level and some clinical parameters in tumour samples (T, N and G). Our study demonstrates that miR-3613-3p overexpression is involved in the tumour progression of OSCC. This indicates that miR-3613-3p possesses potential prognostic values.

Keywords: MDM2; OSCC; margin; miR-3613-3p; miR-3658; miR-371b-5p; oral squamous cell carcinoma; tumour.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
The relative miR-3613-3p expression level in tumour and margin samples.
Figure 2
Figure 2
The relative miR-3613-3p expression level in non-smokers and smokers.
Figure 3
Figure 3
The relative miR-3613-3p expression level in patients with T2 and T3.
Figure 4
Figure 4
The relative miR-3613-3p expression level in patients with N0 and N2.
Figure 5
Figure 5
The relative miR-3613-3p expression level in patients with G1 and G2.
Figure 6
Figure 6
The relative MDM2 gene expression level in the tumour and margin samples.
See this image and copyright information in PMC

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