Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2023 Nov 19;13(22):3571.
doi: 10.3390/ani13223571.

Beyond Canine Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells Transplantation: An Update on Their Secretome Characterization and Applications

Barbara Merlo  1   2 , Eleonora Iacono  1   2
Affiliations
Review

Beyond Canine Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells Transplantation: An Update on Their Secretome Characterization and Applications

Barbara Merlo et al. Animals (Basel). .

Abstract

A dog is a valuable animal model and concomitantly a pet for which advanced therapies are increasingly in demand. The characteristics of mesenchymal stem/stromal cells (MSCs) have made cell therapy more clinically attractive. During the last decade, research on the MSC therapeutic effectiveness has demonstrated that tissue regeneration is primarily mediated by paracrine factors, which are included under the name of secretome. Secretome is a mixture of soluble factors and a variety of extracellular vesicles. The use of secretome for therapeutic purposes could have some advantages compared to cell-based therapies, such as lower immunogenicity and easy manufacturing, manipulation, and storage. The conditioned medium and extracellular vesicles derived from MSCs have the potential to be employed as new treatments in veterinary medicine. This review provides an update on the state-of-the-art characterization and applications of canine adipose tissue-derived MSC secretome.

Keywords: adipose tissue; canine; conditioned medium; extracellular vesicles; mesenchymal stromal cells; secretome.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Graphical representation of the isolation and clinical applications of canine adipose tissue-derived mesenchymal stem/stromal cells (cAT-MSCs).
Figure 2
Figure 2
Classification of extracellular vesicles (EVs) according to their size and cell of origin. Different from the other subtypes of EVs, exomeres are defined as small (<50 nm), non-membranous extracellular nanoparticles. Exosomes originate as intraluminal vesicles while other EVs derive from the outward budding of the plasma membrane.
Figure 3
Figure 3
Representation of the identified soluble factors of secretome from canine adipose tissue-derived mesenchymal stem/stromal cells.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Hoffman A.M., Dow S.W. Concise Review: Stem Cell Trials Using Companion Animal Disease Models. Stem Cells. 2016;34:1709–1729. doi: 10.1002/stem.2377. - DOI - PubMed
    1. Harman R.M., Marx C., Van de Walle G.R. Translational Animal Models Provide Insight into Mesenchymal Stromal Cell (MSC) Secretome Therapy. Front. Cell Dev. Biol. 2021;9:654885. doi: 10.3389/fcell.2021.654885. - DOI - PMC - PubMed
    1. Dominici M., Le Blanc K., Mueller I., Slaper-Cortenbach I., Marini F.C., Krause D.S., Deans R.J., Keating A., Prockop D.J., Horwitz E.M. Minimal Criteria for Defining Multipotent Mesenchymal Stromal Cells. The International Society for Cellular Therapy Position Statement. Cytotherapy. 2006;8:315–317. doi: 10.1080/14653240600855905. - DOI - PubMed
    1. Stewart M.C., Stewart A.A. Mesenchymal Stem Cells: Characteristics, Sources, and Mechanisms of Action. Vet. Clin. Equine Pract. 2011;27:243–261. doi: 10.1016/j.cveq.2011.06.004. - DOI - PubMed
    1. Ullah M., Liu D.D., Thakor A.S. Mesenchymal Stromal Cell Homing: Mechanisms and Strategies for Improvement. iScience. 2019;15:421–438. doi: 10.1016/j.isci.2019.05.004. - DOI - PMC - PubMed

LinkOut - more resources