Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Nov 9;24(22):16126.
doi: 10.3390/ijms242216126.

Nanoparticles Based on Silver Chloride and Bambusuril[6] for the Fine-Tuning of Biological Activity

Pana Turebayeva  1 , Venera Luchsheva  2 , Dmitriy Fedorishin  2 , Rakhmetulla Yerkassov  1 , Abdigali Bakibaev  2 , Saltanat Bolysbekova  3 , Tokzhan Tugambayeva  4 , Samal Sergazina  5 , Nurgul Nurmukhanbetova  5
Affiliations

Nanoparticles Based on Silver Chloride and Bambusuril[6] for the Fine-Tuning of Biological Activity

Pana Turebayeva et al. Int J Mol Sci. .

Abstract

The prevalence of numerous infectious diseases has emerged as a grave concern within the realm of healthcare. Currently, the issue of antibiotic resistance is compelling scientists to explore novel treatment approaches. To combat these infectious diseases, various treatment methods have been developed, harnessing cutting-edge disinfecting nanomaterials. Among the range of metallic nanoparticles employed in medicine, silver nanoparticles (AgNPs) stand out as both highly popular and well-suited for the task. They find extensive utility in cancer diagnosis and therapies and as effective antibacterial agents. The interaction between silver and bacterial cells induces significant structural and morphological alterations, ultimately leading to cell demise. In this study, nanoparticles based on silver and bambusuril[6] (BU[6]) were developed for the first time. These NPs can be used for different biomedical purposes. A simple, single-step, and effective synthesis method was employed to produce bambusuril[6]-protected silver chloride nanoparticles (BU[6]-Ag/AgCl NPs) through the complexation of BU[6] with silver nitrate. The NPs were characterized using X-ray phase analysis (XPS), infrared spectroscopy (IR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). When the SEM images were examined, it was seen that the synthesized BU[6]-Ag/AgCl NPs were distributed with homogeneous sizes, and the synthesized NPs were mostly spherical and cubic. The EDS spectra of BU[6]-Ag/AgCl NPs demonstrated the presence of Ag, Cl, and all expected elements. BU[6]-Ag/AgCl NPs showed high antibacterial activity against both E. coli and S. aureus bacteria.

Keywords: MTT-test; antibacterial activity; bambusuril[6]; silver chloride nanoparticles; supramolecular chemistry; thermal analysis.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Scheme 1
Scheme 1
Synthesis of bambusuril[6].
Figure 1
Figure 1
The X-ray crystal structure of BU[6] (A) cross-section of BU[6], (B) cell parameters. The interior cavity of bambusuril[6] accommodates Cl−.
Figure 2
Figure 2
The scheme shows the synthesis of BU[6]-Ag/AgCl NPs.
Figure 3
Figure 3
UV-visible spectrophotometry of BU[6]-AgCl NPs.
Figure 4
Figure 4
SEM image of synthesized BU[6]-Ag/AgCl NPs.
Figure 5
Figure 5
EDS analysis of synthesized BU[6]-Ag/AgCl NPs.
Figure 6
Figure 6
XRD patterns of BU[6], AgNO3, BU[6]-Ag/AgCl NPs.
Figure 7
Figure 7
FTIR spectra of BU[6], AgNO3, and BU[6]-Ag/AgCl.
Figure 8
Figure 8
Thermal analysis of BU[6]-Ag/AgCl.
Figure 9
Figure 9
Thermal analysis for BU[6] and BU[6]-Ag/AgCl.
Figure 10
Figure 10
Micrograph of the culture of human mononuclear cells incubated with (A,C) BU[6] and (B,D) BU[6]-Ag/AgCl NPs. The incubation durations were 24 h (A,B) and 144 h (C,D). A total of 1 mL of cell medium containing cells at a concentration of 1 × 106 cells and 10 µL of the sample suspension (BU[6]-Ag/AgCl NPs; BU[6]) was added to each well of a 24-well plate. Black arrows—live cells, dotted arrows—live cells.
Figure 11
Figure 11
MTT test used for evaluating the cell viability levels of different donors after the incubation of BU[6] and BU[6]-Ag/AgCl NPs for 144 h.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Cassano D., Mapanao A.-K., Summa M., Vlamidis Y., Giannone G., Santi M., Guzzolino E., Pitto L., Poliseno L., Bertorelli R., et al. Biosafety and Biokinetics of Noble Metals: The Impact of Their Chemical Nature. ACS Appl. Bio Mater. 2019;2:4464–4470. doi: 10.1021/acsabm.9b00630. - DOI - PubMed
    1. Sondi I., Salopek-Sondi B. Silver Nanoparticles as Antimicrobial Agent: A Case Study on E. coli as a Model for Gram-Negative Bacteria. J. Colloid Interface Sci. 2004;275:177–182. doi: 10.1016/j.jcis.2004.02.012. - DOI - PubMed
    1. Sharma V.K., Yngard R.A., Lin Y. Silver Nanoparticles: Green Synthesis and Their Antimicrobial Activities. Adv. Colloid Interface Sci. 2009;145:83–96. doi: 10.1016/j.cis.2008.09.002. - DOI - PubMed
    1. Gurunathan S., Kalishwaralal K., Vaidyanathan R., Venkataraman D., Pandian S.R.K., Muniyandi J., Hariharan N., Eom S.H. Biosynthesis, Purification and Characterization of Silver Nanoparticles Using Escherichia coli. Colloids Surf. B Biointerfaces. 2009;74:328–335. doi: 10.1016/j.colsurfb.2009.07.048. - DOI - PubMed
    1. Panáček A., Kolář M., Večeřová R., Prucek R., Soukupová J., Kryštof V., Hamal P., Zbořil R., Kvítek L. Antifungal Activity of Silver Nanoparticles against Candida spp. Biomaterials. 2009;30:6333–6340. doi: 10.1016/j.biomaterials.2009.07.065. - DOI - PubMed

Grants and funding