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. 2023 Nov 10;28(22):7521.
doi: 10.3390/molecules28227521.

Resveratrol, ε-Viniferin, and Vitisin B from Vine: Comparison of Their In Vitro Antioxidant Activities and Study of Their Interactions

Biranty Sy  1 , Stéphanie Krisa  1 , Tristan Richard  1 , Arnaud Courtois  1   2
Affiliations

Resveratrol, ε-Viniferin, and Vitisin B from Vine: Comparison of Their In Vitro Antioxidant Activities and Study of Their Interactions

Biranty Sy et al. Molecules. .

Abstract

The control of oxidative stress with natural active substances could limit the development of numerous pathologies. Our objective was to study the antiradical effects of resveratrol (RSV), ε-viniferin (VNF), and vitisin B (VB) alone or in combination, and those of a standardized stilbene-enriched vine extract (SSVE). In the DPPH-, FRAP-, and NO-scavenging assays, RSV presented the highest activity with an IC50 of 81.92 ± 9.17, 13.36 ± 0.91, and 200.68 ± 15.40 µM, respectively. All binary combinations resulted in additive interactions in the DPPH- and NO-scavenging assays. In the FRAP assay, a synergic interaction for RSV + VNF, an additive for VNF + VB, and an antagonistic for RSV + VB were observed. The ternary combination of RSV + VNF + VB elicited an additive interaction in the DPPH assay and a synergic interaction in the FRAP- and NO-scavenging assays. There was no significant difference between the antioxidant activity of the SSVE and that of the combination of RSV + VNF. In conclusion, RSV presented the highest effects, followed by VNF and VB. The interactions revealed additive or synergistic effects, depending on the combination of the stilbenes and assay.

Keywords: antioxidant; interactions; resveratrol; stilbenes; vitisin B; ε-viniferin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 7
Figure 7
Summary of IC50 values for resveratrol in the DPPH assay according to the literature [4,12,13,14,15,16,21,25].
Figure 1
Figure 1
Molecular structures of stilbenes isolated from Vitis vinifera vine shoots. (A) Resveratrol; (B) ε-viniferin; (C) vitisin B.
Figure 2
Figure 2
Antioxidant capacity of individual stilbenes (RSV, resveratrol; VNF, ε-viniferin; VB, vitisin B). (A) DPPH assay; (B) FRAP assay; (C) NO-scavenging assay.
Figure 3
Figure 3
DPPH radical reduction and antioxidant capacity of individual stilbenes (RSV, resveratrol; VNF, ε-viniferin; VB, vitisin B) and their equimolar combinations. The 100% marker represents the DPPH alone, which was used as a control. The result of the representative experiment is shown. Values are expressed as mean ± standard deviation (n = 3). Data were analyzed with an ANOVA (p < 0.05). Each letter (a, b, and c) indicates that the combinations were statistically different from the individual compounds (a for resveratrol, b for VNF, and c for VB) according to a post hoc Tukey comparison test at p < 0.05.
Figure 4
Figure 4
Ferric iron reduction capacity of individual stilbenes (RSV, resveratrol; VNF, ε-viniferin; VB, vitisin B) and their equimolar combinations. The 100% marker represents the Trolox that was used as a positive control. The result of the representative experiment is shown. Values are expressed as mean ± standard deviation (n = 4). Data were analyzed with an ANOVA (p < 0.05). Each letter (a, b, and c) indicates that the combinations were statistically different from the individual compounds (a for resveratrol, b for VNF, and c for VB) according to a post hoc Tukey comparison test at p < 0.05.
Figure 5
Figure 5
NO-scavenging capacity of individual stilbenes (RSV, resveratrol; VNF, ε-viniferin; VB, vitisin B) and their equimolar combinations. The 100% marker represents the SNP alone, which was used as a positive control. The result of the representative experiment is illustrated. Values are expressed as mean ± standard deviation (n = 4). Data were analyzed with an ANOVA (p < 0.05). Each letter (a, b, and c) indicates that the combinations were statistically different from the individual compounds (a for resveratrol, b for VNF, and c for VB) according to a post hoc Tukey comparison test at p < 0.05.
Figure 6
Figure 6
Comparison of the antioxidant activities between the combination (RSV + VNF) and the standardized stilbene-enriched vine extract (SSVE). Data were analyzed with an ANOVA (p < 0.05). The letter a indicates that the combination was statistically different from the SSVE according to a post hoc Tukey comparison test at p < 0.05.
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