Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Nov 11;12(22):3827.
doi: 10.3390/plants12223827.

Application of Bacillus spp. Phosphate-Solubilizing Bacteria Improves Common Bean Production Compared to Conventional Fertilization

Antonia Erica Santos de Souza  1 , Vinicius Augusto Filla  1 , João Paulo Morais da Silva  1 , Marcelo Rodrigues Barbosa Júnior  1 , Christiane Abreu de Oliveira-Paiva  2 , Anderson Prates Coelho  1 , Leandro Borges Lemos  1
Affiliations

Application of Bacillus spp. Phosphate-Solubilizing Bacteria Improves Common Bean Production Compared to Conventional Fertilization

Antonia Erica Santos de Souza et al. Plants (Basel). .

Abstract

The use of phosphate-solubilizing bacteria (PSB) can be a sustainable strategy to increase phosphorus availability and promote satisfactory crop yields. The objective of this study was to evaluate whether inoculation with PSB in common bean increases (i) growth, (ii) nutrition, (iii) yield, and (iv) grain quality, and (v) reduces the chemical phosphorus application dose to obtain maximum yields. The experiment was conducted in an Oxisol using a randomized block design in a 4 × 4 factorial scheme, with four replicates, using the cultivar IAC 2051. The first factor was four doses of P2O5 (0, 20, 40 and 60 kg ha-1), and the second factor was four doses of PSB (0, 100, 200 and 300 mL ha-1). For leaf area and leaf chlorophyll content, the association of PSB inoculation with a P2O5 dose of 40 kg ha-1 promoted the best conditions for the common bean. P2O5 application increased yield by 79 kg ha-1 for each 10 kg ha-1 added. PSB inoculation at a dose of 192 mL ha-1 promoted P export of 15.3 kg ha-1, and the PSB dose of 159 mL ha-1 increased yield by 389 kg ha-1 (12%) compared to the control. Grain quality remained within the standards required by the consumer market, being little affected by the treatments. Improvements in common bean growth and nutritional and physiological status promoted by P2O5 application and PSB were essential in increasing yield, so these are sustainable production strategies.

Keywords: Bacillus megaterium; Bacillus subtilis; Phaseolus vulgaris L.; phosphorus; sustainability.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
P content in leaves (PCL, (A)), dry matter (PCDM, (B)) and grains (PCG, (C)) of common bean as a function of P2O5 doses.
Figure 2
Figure 2
P content in the dry matter (PCDM, (A)), P accumulation in the dry matter (PADM, (B)) and P export (C) by common beans as a function of phosphate-solubilizing bacteria (PSB).
Figure 3
Figure 3
Decomposition of the interaction between P2O5 doses and phosphate solubilizing bacteria (PSB) doses for chlorophyll a (Chl a, (A,B)), chlorophyll b (Chl b, (C,D)) and carotenoids (Cx + c, (E,F)) in common bean plants in the vegetative stage (V4).
Figure 4
Figure 4
Chlorophyll a (Chl a, (A)), chlorophyll b (Chl b, (B)) and chlorophyll total (Chl total, (C)) in common bean plants in stage R8 as a function of P2O5 doses.
Figure 5
Figure 5
Chlorophyll content index (CCI) in common bean plants as a function of P2O5 doses.
Figure 6
Figure 6
Interactions between P2O5 doses (A) and phosphate-solubilizing bacteria doses (PSB, (B)) for leaf area (LA) of common beans.
Figure 7
Figure 7
Number of pods per plant (NPP, (A)) and number of grains per pod (NGP, (B)) as a function of phosphate-solubilizing bacteria (PSB) doses.
Figure 8
Figure 8
Dry matter (DM, (A,B)) and yield (YLD, (C,D)) of common bean plants as a function of P2O5 doses and phosphate-solubilizing bacteria (PSB) doses.
Figure 9
Figure 9
Principal component analysis for spatialization of treatments and variables in two dimensions (biplot). PC: principal component; P: P2O5 doses; PSB: phosphate-solubilizing bacteria; YLD: grain yield; NPP: number of pods per plant; NGP: number of grains per pod; HGW: hundred-grain weight; DM: aerial part dry matter; CCI: chlorophyll content index; Chl total: total chlorophyll content in the leaves; PCL: P content in the leaves; PCG: P content in the grains.
See this image and copyright information in PMC

References

    1. Almeida C.P., Paulino J.F.C., Carbonell S.A.M., Chiorato A.F., Song Q., Vittori V.D., Rodriguez M., Papa R., Benchimol-Reis L.L. Genetic diversity, population structure, and andean introgression in Brazilian common bean cultivars after half a century of genetic breeding. Genes. 2020;11:1298. doi: 10.3390/genes11111298. - DOI - PMC - PubMed
    1. Castro-Guerrero N.A., Isidra-Arellano M.C., Mendoza-Cozatl D.G., Valdés-López O. Common Bean: A Legume Model on the Rise for Unraveling Responses and Adaptations to Iron, Zinc, and Phosphate Deficiencies. Front. Plant Sci. 2016;7:391–417. doi: 10.3389/fpls.2016.00600. - DOI - PMC - PubMed
    1. Vougeleka V., Savvas D., Ntatsi G., Ellinas G., Zacharis A., Iannetta P.P.M., Mylona P., Saitanis C.J. Impact of the rootstock genotype on the performance of grafted common bean (Phaseolus vulgaris L.) cultivars. Sci. Hortic. 2023;311:111821. doi: 10.1016/j.scienta.2022.111821. - DOI
    1. Hungria M., Vargas M.A. Environmental factors affecting N2 fixation in grain legumes in the tropics, with an emphasis on Brazil. Field Crops Res. 2000;65:151–164. doi: 10.1016/S0378-4290(99)00084-2. - DOI
    1. Barros R.L.N., Oliveira L.B., Magalhães W.B., Médici L.O., Pimentel C. Interaction of biological nitrogen fixation with sowing nitrogen fertilization on common bean in the two seasons of cultivation in Brazil. J. Plant Nutr. 2018;41:774–781. doi: 10.1080/01904167.2018.1426016. - DOI