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. 2023 Nov 25:78:100311.
doi: 10.1016/j.clinsp.2023.100311. eCollection 2023.

miRNA-143 expression is associated with inflammation and time of exposure to amniotic fluid in experimental gastroschisis

Ana Maria Bicudo Diniz  1 , Igor José Nogueira Gualberto  2 , Luiza Almeida Lima  3 , Mucio Luiz de Assis Cirino  1 , Rodrigo Kendi Murakami  2 , Bella Luna Colombini Ishikiriama  2 , Rodrigo Ruano  3 , Luiz Fernando Ferraz da Silva  4 , Daniela Tirapelli  1 , Lourenço Sbragia  5
Affiliations

miRNA-143 expression is associated with inflammation and time of exposure to amniotic fluid in experimental gastroschisis

Ana Maria Bicudo Diniz et al. Clinics (Sao Paulo). .

Abstract

Objective: Gastroschisis (GS) is a congenital anomaly in the abdominal wall with the intestinal loops exiting laterally to the umbilicus. The contact of the loops with Amniotic Fluid (AF) causes an inflammatory process in the exposed part, leading to an extended hospital stay and an increased risk of morbidity due to alterations related to intestinal motility. The authors aimed to evaluate the time of exposure to the AF in the experimental GS and to search for potential biomarkers of intestinal inflammation by measuring microRNAs.

Methods: Rat fetuses were divided into three groups: a) CONTROL, b) GS reared on day 18 (GS = 18), and c) GS reared on day 19.5 (GS = 19) (term = 22 days). On day 21.5, the fetuses were removed for biometric parameters and biochemical analyses: 1) Biometrics: Body and Intestinal Weight (BW, IW), and intestinal-body weight ratio (IW/BW); 2) Descriptive histopathology and 3) miR-143 quantification by real-time Polymerase Chain Reaction (PCR).

Results: BW was higher in CONTROL than GS 18 and G19 (p < 0.05). IW, IW/BW, intestinal water, and mRNA-143 were higher in GS 18 and GS 19 than in CONTROL, and GS 18 was higher than GS 19 (p < 0.05). The average of the inflammation score from the intestinal wall with mucosal inflammation and intra-epithelial lymphocytes shows worst in GS 18 and GS 19 vs. CONTROL (p < 0.05).

Conclusions: The tissue expression of mRNA-143 and the morphological changes in the intestine of GS worsened according to the time of exposure to AF, which could be a possible marker of fetal intestinal damage.

Keywords: Amniotic fluid; Gastroschisis; Inflammation; Rat; mRNA-143.

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Conflict of interest statement

Declaration of Competing Interest The authors declare no conflicts of interest.

Figures

Fig 1
Fig. 1
Represents the biometric parameters and comparisons among the groups with (SEM).
Fig 2
Fig. 2
Represents the amount of intestinal water after two different times of exposure of amniotic fluid after creation of gastroschisis in rat model. * p < 0.05 GS 18 × Control, and ** p < 0.005 and GS 18 × GS 19.
Fig 3
Fig. 3
Represents the macroscopic view of gastrochisis at different gestational ages. (A) Neonate control; (B) Neonate with GS 18 shows the intestinal loops with edema and inflammatory aspect and (C) Neonate with GS 19 shows less edema and a decrease of inflammatory.
Fig 4
Fig. 4
Represents the histological findings of the gastroschisis villus indifferent gestational ages. Cross sections of intestinal villi obtained from GS 18 (A), GS 19 (B) and Controls (C). Note the increased thickness of the muscle layer of the bowel of the fetuses with gastroschisis. Scale bar = 100 µm.
Fig 5
Fig. 5
Represents the inflammatory findings of the gastroschisis villus indifferent gestational ages. Cross sections of intestinal villi obtained from GS 18 (A), GS 19 (B) and Controls (C). Note the increased number of inflammatory cells in the mucosa of the bowel of the fetuses with gastroschisis groups. Scale bar = 100 micrometer.
Fig 6
Fig. 6
Shows the amount of miR-143 in the intestinal tissue of fetal rats.
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