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. 1986 Jul 15;237(2):613-6.
doi: 10.1042/bj2370613.

Characterization of haem disorder by circular dichroism

Characterization of haem disorder by circular dichroism

H S Aojula et al. Biochem J. .

Abstract

Native and reconstituted myoglobin were prepared and their c.d. spectra recorded in the Soret region. Time-dependent changes in dichroism following reconstitution were observed and related to haem orientational disorder. Comparative c.d. studies, in agreement with n.m.r. studies, reveal that the degree and nature of this disorder are species-dependent.

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