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. 2023 Dec;13(12):415.
doi: 10.1007/s13205-023-03808-4. Epub 2023 Nov 24.

Exploring Bacillus amyloliquefaciens strain OM81 for the production of polyhydroxyalkanoate (PHA) bioplastic using olive mill wastewater

Samar Bacha  1 , Fatma Arous  2 , Emna Chouikh  1 , Atef Jaouani  2 , Maher Gtari  1   3   4 , Khaled Charradi  5 , Hamadi Attia  4 , Dorra Ghorbel  1   4
Affiliations

Exploring Bacillus amyloliquefaciens strain OM81 for the production of polyhydroxyalkanoate (PHA) bioplastic using olive mill wastewater

Samar Bacha et al. 3 Biotech. 2023 Dec.

Abstract

In this study, bacterial strains isolated from olive oil mill wastewater assigned to Bacillus (n = 4) and Klebsiella (n = 1) genera, were evaluated for their ability to accumulate intracellular PHA granules using Sudan Black staining. A maximum PHA production of 0.14 g/L (i.e., 30.2% wt./wt. in dry biomass) was observed in Bacillus amyloliquefaciens strain OM81 after 72 h of incubation in the presence of 2% glucose (synthetic medium). To reduce bioplastic production costs and recover a polluting product, olive mill wastewater was tested as a carbon source. In this context, the maximum growth (1.45 g/L) was observed in the presence of 50% olive mill wastewater. After extracting the biopolymers with chloroform, quantitative and qualitative analyses were conducted using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). FTIR showed an absorption band at 1730 cm-1 assigned to the elongation of the PHB carbonyl groups. This approach offers a dual benefit of reducing pollution and bioplastic production costs. The Bacillus amyloliquefaciens strain OM81 showed promising results for PHAs production, making it a potential candidate for further investigation.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03808-4.

Keywords: Bacillus amyloliquefaciens; Bioplastic; Fermentation; Olive mill wastewater; PHA.

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Conflict of interest statement

Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare that they have no conflict of interest in the publication.

Figures

Fig. 1
Fig. 1
UV–Vis spectrophotometer scanning spectrum of PHA compounds extracted from bacterial strains grown in glucose-based medium of the five bacterial strains: (◆) B. thioparans OM39, (◊) B. thuringiensis OM55, (△) B. cereus OM75, (▲) B. amyloliquefaciens OM81, and (□) K. oxytoca OM91
Fig. 2
Fig. 2
UV–Vis spectrophotometer scanning spectrum of PHA compounds extracted from the strain Bacillus amyloliquefaciens OM81 grown in media where glucose was replaced by OMW at different concentrations: (◆) 25%, (◊) 50%, (△) 75%, and (▲) 100% (v/v)
Fig. 3
Fig. 3
FTIR spectrum of PHA extracted from the strain Bacillus amyloliquefaciens OM81 grown in glucose-based medium
Fig. 4
Fig. 4
TGA (A) and DTG (B) curves of PHA extracted from the strain Bacillus amyloliquefaciens OM81 grown in glucose-based medium
See this image and copyright information in PMC

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