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. 1986 Sep 1;238(2):617-9.
doi: 10.1042/bj2380617.

Aldehyde dehydrogenase catalyses acetaldehyde formation from 4-nitrophenyl acetate and NADH

K M Loomes, T M Kitson

Aldehyde dehydrogenase catalyses acetaldehyde formation from 4-nitrophenyl acetate and NADH

K M Loomes et al. Biochem J. .

Abstract

Incubation of sheep liver cytoplasmic aldehyde dehydrogenase with the substrate 4-nitrophenyl [14C]acetate in the presence of NADH leads to the formation of 14C-labelled acetaldehyde. This observation strongly supports the idea that the esterase and dehydrogenase activities of the enzyme occur at the same site and involve the intermediacy of a common acyl-enzyme.

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