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. 2023 Nov 1;24(11):3875-3882.
doi: 10.31557/APJCP.2023.24.11.3875.

Prognostic Value of Immunohistochemical Expression of MTAP and AKIP1 in IDH1 Mutant Astrocytoma

Mona Mostafa Ahmed  1 , Mohammed A Lateef  2 , Amira Elwan  3 , Enas M Fouad  1 , Dalia Hamouda Elsayed  4 , Hanim M Abdelnour  5 , Asmaa Abdullatif  1
Affiliations

Prognostic Value of Immunohistochemical Expression of MTAP and AKIP1 in IDH1 Mutant Astrocytoma

Mona Mostafa Ahmed et al. Asian Pac J Cancer Prev. .

Abstract

Background: Definite treatment for glioma is not exist, and with increased drug resistance, more effort should be paid to identify new prognostic biomarkers and molecular targets for therapy for glioma patients.

Aim: The current study aimed to evaluate the immunohistochemical (IHC) expression of MTAP and A-Kinase Interacting Protein 1 (AKIP1) in astrocytoma and to investigate their association with the clinicopathological characters of these cases.

Methods: Totally 66 cases of astrocytoma patients involved in this study. Cases underwent tumor resection and tissue sections were stained with MTAP, AKIP1 and IDH1 by IHC and evaluated in different grades of astrocytoma and their association with survival and response to therapy was investigated.

Results: High AKIP1 expression was positively correlated with treatment resistance and progressive disease. Positive IDH and retained MTAP expressions had shown better treatment response rather than negative IDH and lost MTAP. High AKIP, negative IDH and loss of MTAP expressions were significantly associated with poor survival outcome.

Conclusion: Irrespective to grade and IDH status, the loss of MTAP immunoreactivity and high AKIP1 expression are predictive factors in astrocytoma, and they may be used as a biomarker for guiding astrocytoma management and prognosis surveillance.

Keywords: AKIP1; Isocitrate dehydrogenase1; MTAP; astrocytoma; survival.

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Conflict of interest statement

No competing interests.

Figures

Figure 1
Figure 1
1 IDH1 Immunohistochemistry: a- a case of anaplastic astrocytoma all cells are not stained (x400). b- diffuse astrocytoma IDH stains scattered cells and most cells of the tumor are not labeled (x400). C. diffuse astrocytoma showing IDH positive immunostaining in all tumor cells immunostaining in all tumor cells (x200)
Figure 2
Figure 2
a. MTAP immunohistochemistry demonstrating the loss of cytoplasmic MTAP staining while the internal control is positively labelled. b. A case of IDH-mutant astrocytoma retaining cytoplasmic MTAP staining (x400)
Figure 3
Figure 3
A-kinase Interacting Protein 1 (AKIP1) Expression in Glioma. The representative image of AKIP1 a. negative, b. mild expression, c. moderate and d. high expression in astrocytoma (x400)
Figure 4
Figure 4
Kaplan Meier Plots, Left panel: progression free survival, right panel :overall survival stratified by MTAP and AKIP expression
See this image and copyright information in PMC

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