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. 2024 Oct;50(5):2013-2024.
doi: 10.1007/s10695-023-01273-7. Epub 2023 Nov 29.

In vitro post-ovulatory oocyte ageing in grass carp Ctenopharyngodon idella affects H4K12 acetylation pattern and histone acetyltransferase activity

Azin Mohagheghi Samarin #  1 , Azadeh Mohagheghi Samarin #  2 , Swapnil Gorakh Waghmare  2 , Marianne Danielsen  3   4   5 , Hanne Søndergård Møller  3 , Tomáš Policar  2 , Otomar Linhart  2 , Trine Kastrup Dalsgaard  3   4   5
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In vitro post-ovulatory oocyte ageing in grass carp Ctenopharyngodon idella affects H4K12 acetylation pattern and histone acetyltransferase activity

Azin Mohagheghi Samarin et al. Fish Physiol Biochem. 2024 Oct.

Abstract

Delayed fertilization leads to the ageing of post-ovulatory oocytes and reduces the developmental competence of arising embryos. Little information is available about the molecular processes during fish oocyte ageing. The current study investigated the functional consequences of oocyte ageing in grass carp Ctenopharyngodon idella embryos. In addition, the dynamics of selected post-transcriptionally modified histones (acetylation of H3K9, H3K14, H4K5, H4K8, H4K12, and H4K16) were analyzed during oocyte ageing. Ovulated oocytes were aged in vitro for 4 h in the laboratory incubator at 20 °C and studied for selected post-translational modification of histones. In addition, histone acetyltransferase activity was investigated as an important regulator of histone acetylation modification. The results indicated a significant decrease in oocyte fertilizing ability through 1 h of post-ovulatory ageing, and a complete loss of egg fertilizing abilities was detected at 4-h aged oocytes. Furthermore, post-ovulatory oocyte ageing for 1 and 4 h led to decreased levels of H4K12 acetylation. The activity of histone acetyltransferases increased significantly after ageing of the oocytes for 30 h in vitro. This modification may partly contribute to explaining the failures of egg viability and embryo development in the offspring from the aged oocytes. The results are the first to report histone modifications as a crucial epigenetic regulator during oocyte ageing in fish and might also benefit other vertebrates.

Keywords: Ctenopharyngodon Idella; Epigenetics; Fertilization; HAT activity; Histone acetylation; Ovulation.

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Conflict of interest statement

Declarations This study was conducted according to the guidelines of the EU-harmonized Animal Welfare Act of the Czech Republic and approved by the expert committee of the Institutional Animal Care and Use Committee (IACUC) of the University of South Bohemia, Czech Republic. According to the Czech National Directive, the unit is licensed (No. 16OZ19179/2016-17214) (Law against Animal Cruelty, No. 246/1992). The co-authors of this study who were involved in manipulating fish hold certificates giving them the right to work with laboratory animals according to Section 15d, paragraph 3, of Act No. 246/1992 Coll. The study did not involve endangered or protected species. To examine ovulation and to collect gametes, brood fish were anaesthetized with a 0.03 ml/L clove oil water bath to enhance animal welfare and minimize stress. None of the experimental animals, either broodfish or larvae, were sacrificed during this study. Competing interests The authors declare no competing interests.

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