Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Oct 8:9:80-87.
doi: 10.1016/j.ijregi.2023.09.008. eCollection 2023 Dec.

A One Health approach based on genomics for enhancing the Salmonella enterica surveillance in Colombia

Johan F Bernal  1 , Paula L Díaz  2 , Blanca M Perez-Sepulveda  3 , María Fernanda Valencia-Guerrero  1 , Viviana Clavijo  4 , Magdalena Weisner  2 , Lucy Angeline Montaño  2 , Stefany A Arevalo  1 , Ingrid Maribel León  5 , Luis Ricardo Castellanos  6 , Anthony Underwood  7 , Carolina Duarte  2 , Silvia Argimón  7 , Jaime Moreno  2 , David Aanensen  7 , Pilar Donado-Godoy  1
Affiliations

A One Health approach based on genomics for enhancing the Salmonella enterica surveillance in Colombia

Johan F Bernal et al. IJID Reg. .

Abstract

Objectives: This study aimed to provide evidence of the domestic benefits of introducing an integrative genomic analysis from the One Health approach in the national surveillance of Salmonella enterica between 1997-2017 in Colombia.

Methods: Data on Salmonella from clinical laboratory-based surveillance between 1997-2017 and from a national cross-sectional study at chicken retail stores in Colombia were compared using a phenotypic, molecular, and genomic approaches. Additional analysis by serovar using single nucleotide polymorphism was developed to increase the resolution of the relatedness between the interfaces.

Results: Locally, the diversity and pathogenic factors of the prevalent S. enterica serovars associated with foodborne disease in Colombia were described using laboratory, pulse field gel electrophoresis, and whole genome sequencing data. For example, the resolution of pulse field gel electrophoresis allowed the description of two main foodborne clusters of Salmonella Enteritidis isolates, which were expanded to eight foodborne clades using whole genome sequencing. Likewise, virulence factors and antimicrobial resistance determinants, and mobile genetic elements that converged in the foodborne clades should be considered a public health concern in Colombia. All results by serovar were compiled in an interactive easy to share report.

Conclusion: Whole genome sequencing is a technology that provides a precise assessment of emerging foodborne risks such as the Salmonella foodborne clades, but it requires an integrative and continued collaboration between the stakeholders across the One Health sectors to promote appropriated actions and policies in public health.

Keywords: Antimicrobial resistance; Foodborne; One health; Salmonella; Virulence; WGS.

PubMed Disclaimer

Conflict of interest statement

The authors have no competing interests to declare.

Figures

Figure 1
Figure 1
Pulsed-field gel electrophoresis clustering of Salmonella Enteritidis from food using DICE similarity index and clustering by UPGMA. Two main clonal clusters were identified in food isolates: Isolates with pattern ICA JEG.X01.0006 and isolates with pattern ICA JEG.X01.0001.
Figure 2
Figure 2
Comparison of unique PFGE patterns of Salmonella Enteritidis from clinical (INS) and food (ICA), using DICE similarity index and clustering by UPGMA. Three clonal relations were observed (DSI 1∙0): cluster-1 (JEG.X01.0001 - JEG.X01.0001), cluster-2 (JEG.X01.0006 - JEG.X01.0038) and cluster-3 (JEG.X01.0008 - JEG.X01.0068).
Figure 3
Figure 3
Geographical distribution and phylogeny inference of Salmonella Enteritidis by clade, source, and year.
Figure 4
Figure 4
SNPs distribution matrix of Salmonella Enteritidis Clades.
See this image and copyright information in PMC

References

    1. Kirk MD, Pires SM, Black RE, Caipo M, Crump JA, Devleesschauwer B, et al. World Health Organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal, and viral diseases, 2010: a data synthesis. PLoS Med. 2015;12 doi: 10.1371/JOURNAL.PMED.1001921. - DOI - PMC - PubMed
    1. Salmonella and Campylobacter in Chicken Meat - Meeting Report . MRA Publications; 2022. p. 19.

    2. Microbiological Risk Assessment Series https://www.fao.org/documents/card/en/c/1ba49045-e062-501c-a32f-6f6cb15cf631/ [accessed 30 January 2022].

    1. INS . 2020. Vigilancia brotes de enfermedades transmitidas por alimentos, Colombia, semana epidemiológica 04 de 2020; pp. 1–5.

    2. BES Boletín Epidemiológico Semanal https://www.ins.gov.co/buscador-eventos/BoletinEpidemiologico/2020_Boletin_epidemiologico_semana_31.pdf [accessed 25 June 2021].

    1. Donado-Godoy P, Castellanos R, León M, Arevalo A, Clavijo V, Bernal J, et al. The establishment of the Colombian integrated program for antimicrobial resistance surveillance (COIPARS): a pilot project on poultry farms, slaughterhouses and retail market. Zoonoses Public Health. 2015;62:58–69. doi: 10.1111/ZPH.12192. - DOI - PubMed
    1. World Health Organization . 2020. GLASS whole-genome sequencing for surveillance of antimicrobial resistanc.https://apps.who.int/iris/handle/10665/334354 [accessed 01 April 2022]

LinkOut - more resources