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. 2023 Nov 13:11:1257200.
doi: 10.3389/fcell.2023.1257200. eCollection 2023.

Time-series transcriptomic profiling of larval exsheathment in a model parasitic nematode of veterinary importance

Nikola Palevich #  1 , Paul H Maclean #  1 , Richard W Scott  1
Affiliations

Time-series transcriptomic profiling of larval exsheathment in a model parasitic nematode of veterinary importance

Nikola Palevich et al. Front Cell Dev Biol. .
No abstract available

Keywords: Haemonchus contortus; exsheathment; parasitic nematode; rumen; transcriptome.

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Conflict of interest statement

Authors NP, PM and RS were employed by AgResearch Limited. The reviewer IM declared a past co-authorship with the author NP to the handling editor.

Figures

FIGURE 1
FIGURE 1
Overview of the study design and experimental procedure. A closed in vitro system that effectively reproduces the two basic components of an anaerobic rumen environment (CO2 and 39°C) was used to trigger exsheathment (xL3) in Haemonchus contortus third-stage infective larvae (iL3) in O2-free CO2 saturated saline solution (A). Scanning electron micrographs of the exsheathment process with scale bars adjusted according to magnification of each image. Time-series RNA-seq analysis of Haemonchus contortus larval exsheathment (B). Comparison of the total numbers of clean sequencing reads versus total population exsheathment of L3 larvae. Total reads represent the sum of both unique and duplicate read pairs. Mean of the total exsheathment percentage (±SEM) at each time point across replicates. Top 100 differentially transcribed genes (DTGs) post exsheathment trigger application (C). Standardised relative abundances of inferred gene transcripts (Value) indicating low to high (red to green) relative gene expression (logFC≥2, FDR≥0.05) at each time point, and single linkage clustering. Three distinct profiles of expression were observed within the time-series RNA-Seq data: ‘prolonged/constant’ (pink), ‘oscillating’ (cyan, ≤25 min) and ‘ramped’ (purple, ≥28 min) expression for the majority of time intervals.
FIGURE 2
FIGURE 2
Time-series functional profiling of larval exsheathment in Haemonchus contortus. For each transcriptomic dataset, only the top level-A (A) and corresponding level-B (B) KEGG pathways are depicted. The total sequencing reads assigned to KEGG categories are averages across each time point.
See this image and copyright information in PMC

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