Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Oct 26;14(11):5994-6015.
doi: 10.1364/BOE.502618. eCollection 2023 Nov 1.

Assessment of power spectral density of microvascular hemodynamics in skeletal muscles at very low and low-frequency via near-infrared diffuse optical spectroscopies

Caterina Amendola  1 , Mauro Buttafava  2 , Talyta Carteano  3 , Letizia Contini  1 , Lorenzo Cortese  4 , Turgut Durduran  4   5 , Lorenzo Frabasile  1 , Claudia Nunzia Guadagno  6 , Umut Karadeinz  4 , Michele Lacerenza  2 , Jaume Mesquida  7 , Shahrzad Parsa  8 , Rebecca Re  1   9 , Diego Sanoja Garcia  3 , Sanathana Konugolu Venkata Sekar  6 , Lorenzo Spinelli  9 , Alessandro Torricelli  1   9 , Alberto Tosi  10 , Udo M Weigel  8 , M Atif Yaqub  4 , Marta Zanoletti  4 , Davide Contini  1
Affiliations

Assessment of power spectral density of microvascular hemodynamics in skeletal muscles at very low and low-frequency via near-infrared diffuse optical spectroscopies

Caterina Amendola et al. Biomed Opt Express. .

Erratum in

Abstract

In this work, we used a hybrid time domain near-infrared spectroscopy (TD-NIRS) and diffuse correlation spectroscopy (DCS) device to retrieve hemoglobin and blood flow oscillations of skeletal muscle microvasculature. We focused on very low (VLF) and low-frequency (LF) oscillations (i.e., frequency lower than 0.145 Hz), that are related to myogenic, neurogenic and endothelial activities. We measured power spectral density (PSD) of blood flow and hemoglobin concentration in four muscles (thenar eminence, plantar fascia, sternocleidomastoid and forearm) of 14 healthy volunteers to highlight possible differences in microvascular hemodynamic oscillations. We observed larger PSDs for blood flow compared to hemoglobin concentration, in particular in case of distal muscles (i.e., thenar eminence and plantar fascia). Finally, we compared the PSDs measured on the thenar eminence of healthy subjects with the ones measured on a septic patient in the intensive care unit: lower power in the endothelial-dependent frequency band, and larger power in the myogenic ones were observed in the septic patient, in accordance with previous works based on laser doppler flowmetry.

PubMed Disclaimer

Conflict of interest statement

The role in the project of all the companies and their employees involved (pioNIRS s.r.l., ASPHALION S.L, BioPixs Ltd, HemoPhotonics S.L.) has been defined by the project objectives, tasks, and work packages and has been reviewed by the European Commission (European Union's Horizon 2020 research and innovation programme, VASCOVID project, grant agreement No. 101016087). ICFO has equity ownership in the spin-off company HemoPhotonics S.L. and UMW is the CEO. TD and UMW are inventors on relevant patents. All the potential financial conflicts of interest and objectivity of research have been monitored by ICFO’s Knowledge & Technology Transfer Department. No financial conflicts of interest were identified. M.B., M.L. A.T., A.T., and D.C. are cofounders of pioNIRS S.r.l., (Italy).

Figures

Fig. 1.
Fig. 1.
Schematic of the probe position during measurements: panel a) forearm, panel b) thenar eminence, panel c) sternocleidomastoid, d) plantar fascia.
Fig. 2.
Fig. 2.
Time traces of percentage variations of tHb, StO2 (left column) and BFI (right column) measured on thenar eminence (panels (a) and (b)), forearm (panels (c) and (d)), sternocleidomastoid (panels (e) and (f)) and plantar fascia (panels (g) and (h)) of one exemplary healthy volunteer.
Fig. 3.
Fig. 3.
Zoom of the power spectral density (PSD) highlighting the spectral components at the three intervals of deoxygenated hemoglobin (HHb, panel (a)), oxygenated hemoglobin (HbO2, panel (b)), total hemoglobin (tHb, panel (c)), tissue oxygen saturation (StO2, panel (d)), averaged among all the participants, in different muscles. Lines are the average values, while shadows are the corresponding standard errors.
Fig. 4.
Fig. 4.
Power spectral density (PSD) of blood flow index (BFI) averaged among all the participants, in different muscles. Lines are the average values, while shadows are the corresponding standard errors.
Fig. 5.
Fig. 5.
Power spectral density (PSD) of HHb (panel (a)), HbO2 (panel (b)), tHb (panel (c)) and StO2 (panel (d)) averaged among all the volunteers (blue line) compared with PSDs measured for the septic patient (yellow line), on the thenar eminence.
Fig. 6.
Fig. 6.
Power spectral density (PSD) of blood flow index (BFI) averaged among all the volunteers (blue line) compared with PSD of BFI measured on a septic patient (yellow line), on the thenar eminence.
Fig. 7.
Fig. 7.
Box plot of absolute and relative areas of PSDs measured on the thenar eminence computed in the three intervals of interest in case of healthy subjects (asterisk), compared to the ones measured in the septic patients (red diamond). The red lines inside the boxes represent the median, the bottom and top edges of the box indicate the 25th and 75th percentiles, for healthy volunteers. The whiskers extend to the most extreme data points not considered outliers.
See this image and copyright information in PMC

References

    1. Dolmatova E. V., Wang K., Mandavilli R., Griendling K. K., “The effects of sepsis on endothelium and clinical implications,” Cardiovasc. Res. 117(1), 60–73 (2021).10.1093/cvr/cvaa070 - DOI - PMC - PubMed
    1. Duranteau J., De Backer D., Donadello K., Shapiro N. I., Hutchings S. D., Rovas A., Legrand M., Harrois A., Ince C., “The future of intensive care: the study of the microcirculation will help to guide our therapies,” Crit. Care 27(1), 190 (2023).10.1186/s13054-023-04474-x - DOI - PMC - PubMed
    1. Al-Qaisi M., Kharbanda R. K., Mittal T. K., Donald A. E., “Measurement of endothelial function and its clinical utility for cardiovascular risk,” Vasc. Health Risk Manage. 4, 647–652 (2008).10.2147/VHRM.S2769 - DOI - PMC - PubMed
    1. Flammer A. J., Anderson T., Celermajer D. S., Creager M. A., Deanfield J., Ganz P., Hamburg N. M., Lüscher T. F., Shechter M., Taddei S., Vita J. A., Lerman A., “The assessment of endothelial function: From research into clinical practice,” Circulation 126(6), 753–767 (2012).10.1161/CIRCULATIONAHA.112.093245 - DOI - PMC - PubMed
    1. Wu B., Credeur D., Fryer S., Stoner L., “The use of shear rate-diameter dose-response curves as an alternative to the flow-mediated dilation test,” Med. Hypotheses 84(2), 85–90 (2015).10.1016/j.mehy.2014.12.014 - DOI - PubMed

LinkOut - more resources