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Review
. 2023 Mar 22;11(3):130-134.
doi: 10.4103/jmau.jmau_108_22. eCollection 2023 Jul-Sep.

Diagnostic Utility of Gram Stain for Oral Smears - A Review

Tharani Vijayakumar  1 , Bose Divya  1 , V Vasanthi  1 , Madhu Narayan  1 , Annasamy Ramesh Kumar  1 , Rajkumar Krishnan  1
Affiliations
Review

Diagnostic Utility of Gram Stain for Oral Smears - A Review

Tharani Vijayakumar et al. J Microsc Ultrastruct. .

Abstract

For rapid and successful treatment of infectious diseases, detection of the presence of microorganisms is essential. Traditional culture-based approaches are limiting and time consuming for microbial identification. The most popular staining technique for identifying Gram-positive and Gram-negative microorganisms in various tissues is called Gram staining. This method is utilized in both clinical practice and research. Gram staining of the oral smears is the preliminary step in the identification of any pathological shift in normal oral microbiota. This review discusses the principle of gram stain emphasizing its significance in diagnostic utility for oral smears.

Keywords: Bacteria; Gram-negative; Gram-positive; microorganisms; oral smear; staining.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Classification of staining
Figure 2
Figure 2
Procedure for staining
Figure 3
Figure 3
Procedure for modified Brown-Brenn method
See this image and copyright information in PMC

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