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. 2023 Oct 30;9(11):e21634.
doi: 10.1016/j.heliyon.2023.e21634. eCollection 2023 Nov.

The behaviour of Escherichia coli and Pseudomonas aeruginosa in bottled mineral water

Michael Schalli  1 , Sabine Platzer  1 , Doris Haas  2 , Franz F Reinthaler  1
Affiliations

The behaviour of Escherichia coli and Pseudomonas aeruginosa in bottled mineral water

Michael Schalli et al. Heliyon. .

Abstract

Microbial contamination of bottled water during the filling and capping procedure is a problem which should be avoided. The examination of the influence of carbon dioxide (CO2) on bacterial growth of Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) in bottled mineral water was the aim of this study. Commercially available glass bottles with plastic screw caps filled with natural mineral water (without additional CO2 "still" (StMW) and with CO2 "sparkling" (SpMW) were obtained from a manufacturer in the province of Styria, Austria. The artificial contamination was performed in the lab by opening the bottle with subsequent addition of a bacterial solution with a defined number of bacteria. For each bacterial strain, 12 bottles were prepared. Samples (100 mL) were taken after a specific number of days, filtrated and placed on Endo Agar for cultivation. After incubation for 24 h bacterial colonies were counted. In this study CO2 addition to bottled water reduced colony forming units of the two investigated bacterial strains over time.

Keywords: Carbon dioxide; Escherichia coli; Food contamination; Mineral water; Pseudomonas aeruginosa; Water supply.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Different appearance of colony-forming units of E. coli (a) and P. aeruginosa (b), on Endo Agar.
Fig. 2
Fig. 2
Example of cfu's for background-flora on Endo Agar and Tryptic soy agar of investigated StMW (a) and SpMW (b) of non-contaminated samples on day one after an incubation time of 24 h at 37 °C.
Fig. 3
Fig. 3
Values of colony-forming units (E. coli) over a period of 31 days. StMW (a), SpMW (b).
Fig. 4
Fig. 4
Values of colony-forming units (P. aeruginosa) over a period of 31 days. StMW (a), SpMW (b).
See this image and copyright information in PMC

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