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. 2023 Nov 7:37:e00344.
doi: 10.1016/j.plabm.2023.e00344. eCollection 2023 Nov.

Detection of respiratory pathogenic bacterial nucleic acid detection by Loop-mediated Isothermal Amplification in patients with bacterial pulmonary infections

Chuanzhu Wang  1 , Ziyun Zhang  1 , Xuedong Wang  1 , Boke Zhang  2
Affiliations

Detection of respiratory pathogenic bacterial nucleic acid detection by Loop-mediated Isothermal Amplification in patients with bacterial pulmonary infections

Chuanzhu Wang et al. Pract Lab Med. .

Abstract

Objective: Nucleic acid testing can accurately and rapidly identify the presence of pathogenic bacteria. In this study, we analyzed respiratory pathogenic bacteria nucleic acids by LAMP (Loop-mediated isothermal amplification) to clarify the clinical application in patients with bacterial pulmonary infections.

Methods: Clinical data and specimens were collected from 99 patients with bacterial pulmonary infections from June 2021 to April 2023. We compared the differences between nucleic acid detection of LAMP and sputum culture. The correlation between inflammation manifestations of pulmonary imaging and the nucleic acid detection of LAMP was compared and analyzed. And the relationship between LAMP and blood inflammatory markers were analyzed.

Results: The positive rate of LAMP using sputum specimens was significantly higher than that of sputum culture (P < 0.05). Pathogenic bacteria in sputum samples are more likely to be detected by LAMP in patients with inflammatory on lung imaging examination. The coincidence rate of elevated PCT and CRP expression with positive LAMP results were 83.87 % and 88.71 %, respectively. Moreover, PCT, CRP and WBC were significantly higher in LAMP positive group than those in negative group (P < 0.05).

Conclusion: Nucleic acid testing of sputum specimens for pathogenic bacteria by LAMP on the basis of imaging examination can provide a rapid and accurate experimental basis for clinical diagnosis of bacterial pulmonary infections.

Keywords: CRP; LAMP; Loop-mediated Isothermal Amplification; PCT; Pulmonary infections; Sputum culture.

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Conflict of interest statement

All authors declare that there are no conflicts of interest.

Figures

Fig. 1
Fig. 1
Respiratory pathogens nucleic acid test operation flow.
Fig. 2
Fig. 2
Expression levels of PCT, WBC and CRP in positive and negative groups of respiratory pathogenic bacteria nucleic acid by LAMP. (A) The expression levels of PCT in the two groups. (B) The expression levels of WBC in the two groups. (C) The expression levels of CRP in the two groups.
See this image and copyright information in PMC

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