Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Dec;9(6):1112-1121.
doi: 10.1002/cre2.822. Epub 2023 Nov 30.

Evaluation of the microleakage of class V composite restoration after cavity treatment with Erbium, CO2 lasers, Papain, and Bromelain enzymes

Farahnaz Sharafeddin  1 , Anahita Fadaei Tabrizi  2
Affiliations

Evaluation of the microleakage of class V composite restoration after cavity treatment with Erbium, CO2 lasers, Papain, and Bromelain enzymes

Farahnaz Sharafeddin et al. Clin Exp Dent Res. 2023 Dec.

Abstract

Objectves: Different surface preparation and treatment methods may have dissimilar effects on the microleakage of composite resin. This study was conducted to determine the deproteinizing effect of 10% bromelain enzyme, 10% papain enzyme, CO2 , and erbium-YAG laser in regard to decrease in the microleakage of composite restorations.

Materials and methods: Thirty teeth were selected and 60 class V cavities were prepared on the lingual and buccal sides. They were divided into six groups (n = 10): Group 1, phosphoric acid gel; Group 2, bromelain enzyme 10%; Group 3, papain enzyme 10%; Group 4, mixed papain and bromelain enzymes 10%; Group 5, CO2 laser; and Group 6, erbium-YAG laser. They were stored in basic fuchsine and dye penetration was evaluated. Kruskal-Wallis and Mann-Whitney tests were used for statistical analysis, p < 0.05 RESULTS: In both occlusal and gingival margins, comparison of microleakage between groups 1, 2, 3, 4, and 5 showed no significant differences (p = 1) and group 6 had a significant difference with other groups (p ˂ 0.001).

Conclusions: Microleakage of composite resin in the dentin surface was not affected significantly using either bromelain or papain 10% enzymes or erbium laser. However, CO2 laser had a negative effect on the enamel and dentin margins and increased the microleakage. Erbium laser showed a better effect than enzymes on microleakage.

Keywords: Bromelain; CO2 laser; Erbium lase; Papain.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Experimental groups.
Figure 2
Figure 2
The class V cavity.
Figure 3
Figure 3
Polishing.
Figure 4
Figure 4
Nonstop cutting machine.
Figure 5
Figure 5
Stereomicroscope.
Figure 6
Figure 6
Displays the sectioned teeth (×40 magnification). (a) Score 0: Phosphoric acid 37% group. (b) score 1: Bromelain enzyme 10% group. (c) score 2: Papain enzyme 10% group. (d) score 3: CO2 laser group.
Figure 7
Figure 7
The scores and percentage of occlusal (dentin margin) microleakage in experimental groups. 0 = no dye penetration. 1 = dye penetration between the restoration and tooth up to one‐half of the distance between the tooth surface and the axial wall. 2 = dye penetration extending beyond one‐half but not reaching the axial wall. 3 = dye penetration reaching the axial.
Figure 8
Figure 8
The scores and percentage of gingival (dentin margin) microleakage in experimental groups. 0 = no dye penetration. 1 = dye penetration between the restoration and tooth up to one‐half of the distance between the tooth surface and the axial wall. 2 = dye penetration extending beyond one‐half but not reaching the axial wall. 3 = dye penetration reaching the axial.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Andermatt, L. , & Özcan, M. (2021). Micro‐shear bond strength of resin composite cement to coronal enamel/dentin, cervical enamel, cementoenamel junction and root cementum with different adhesive systems. Journal of Adhesion Science and Technology, 35, 2079–2093.
    1. Apel, C. , Meister, J. , Götz, H. , Duschner, H. , & Gutknecht, N. (2005). Structural changes in human dental enamel after subablative erbium laser irradiation and its potential use for caries prevention. Caries Research, 39(1), 65–70. - PubMed
    1. Basir, M. M. , Rezvani, M. B. , Chiniforush, N. , & Moradi, Z. (2016). Effect of CO2, Nd:YAG and Er:YAG lasers on microtensile bond strength of composite to bleached‐enamel. The Open Dentistry Journal, 10, 148–157. - PMC - PubMed
    1. Chauhan, K. , Shivanna, V. , & Basavanna, R. (2015). Effect of bromelain enzyme for dentin deproteinization on bond strength of adhesive system. Journal of Conservative Dentistry, 18(5), 360–363. - PMC - PubMed
    1. Chinelatti, M. A. , Ramos, R. P. , Chimello, D. T. , Corona, S. A. M. , Pécora, J. D. , & Dibb, R. G. P. (2006). Influence of Er:YAG laser on cavity preparation and surface treatment in microleakage of composite resin restorations. Photomedicine and Laser Surgery, 24(2), 214–218. - PubMed