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. 2023 Feb 3:8:57.
doi: 10.12688/wellcomeopenres.18893.1. eCollection 2023.

The genome sequence of a beetle-killing wasp, Tiphia femorata (Fabricius, 1775)

Liam M Crowley  1 ; University of Oxford and Wytham Woods Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life programme; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Tree of Life Core Informatics collective; Damon-Lee Pointon  2 ; Darwin Tree of Life Consortium
Affiliations

The genome sequence of a beetle-killing wasp, Tiphia femorata (Fabricius, 1775)

Liam M Crowley et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual female Tiphia femorata (a beetle-killing wasp; Arthropoda; Insecta; Hymenoptera; Tiphilidae). The genome sequence is 276 megabases in span. Most of the assembly (98.73%) is scaffolded into 12 chromosomal pseudomolecules. The complete mitochondrial genome was also assembled and is 22.4 kilobases in length. Annotation of the genome in Ensembl identified 10,470 protein-coding genes.

Keywords: Hymenoptera; Tiphia femorata; beetle killing wasp; chromosomal; genome sequence.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Image of the Tiphia femorata specimen taken prior to preservation and processing.
Figure 2.
Figure 2.. Genome assembly of Tiphia femorata, iyTipFemo1.1: metrics.
The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 276,162,441 bp assembly. The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (38,663,132 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 scaffold lengths (25,801,794 and 16,818,620 bp), respectively. The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the hymenoptera_odb10 set is shown at the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/iyTipFemo1.1/dataset/CALUEP01/snail.
Figure 3.
Figure 3.. Genome assembly of Tiphia femorata, iyTipFemo1.1: GC coverage.
BlobToolKit GC-coverage plot. Scaffolds are coloured by phylum. Circles are sized in proportion to scaffold length. Histograms show the distribution of scaffold length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/iyTipFemo1.1/dataset/CALUEP01/blob.
Figure 4.
Figure 4.. Genome assembly of Tiphia femorata, iyTipFemo1.1: cumulative sequence.
BlobToolKit cumulative sequence plot. The grey line shows cumulative length for all scaffolds. Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/iyTipFemo1.1/dataset/CALUEP01/cumulative.
Figure 5.
Figure 5.. Genome assembly of Tiphia femorata, iyTipFemo1.1: Hi-C contact map.
Hi-C contact map of the iyTipFemo1.1 assembly, visualised in HiGlass. Chromosomes are arranged in size order from left to right and top to bottom. The interactive Hi-C map can be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=eT742vyJSUO1PVEDGgOgZg.
See this image and copyright information in PMC

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