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. 2023 Dec 1;133(23):e173715.
doi: 10.1172/JCI173715.

High-frequency home self-collection of capillary blood correlates IFI27 expression kinetics with SARS-CoV-2 viral clearance

Fang Yun Lim  1   2 , Soo-Young Kim  1 , Karisma N Kulkarni  1 , Rachel L Blazevic  1 , Louise E Kimball  1 , Hannah G Lea  2 , Amanda J Haack  2 , Maia S Gower  2 , Terry Stevens-Ayers  1 , Lea M Starita  3   4 , Michael Boeckh  1   5 , Ollivier Hyrien  1 , Joshua T Schiffer  1   5 , Ashleigh B Theberge  2   6 , Alpana Waghmare  1   7   8
Affiliations

High-frequency home self-collection of capillary blood correlates IFI27 expression kinetics with SARS-CoV-2 viral clearance

Fang Yun Lim et al. J Clin Invest. .
No abstract available

Keywords: COVID-19; Infectious disease; Innate immunity.

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Conflict of interest statement

Conflict of interest: FYL, AJH, and ABT filed patent 17/361,322 (“Fluid transfer system for applications including stabilizing biological fluids”) through the University of Washington on homeRNA.

Figures

Figure 1
Figure 1. Self-blood collection captures temporal alignment between IFI27 and SARS-CoV-2 VL.
(A) Dynamic genes and pathways identified through GAMM and TcGSA, respectively. (B) Spaghetti plots depicting time trends of a dynamic antiviral pathway identified through TcGSA. Colored solid lines represent the scaled median expression of one gene across all participants, and black dotted lines represent the smoothed median of all genes for a given time trend. Colors denote genes in distinct time trend clusters (blue, cluster 1; pink, cluster 2). (C) Pearson’s correlation matrix of genes with ≥0.3 absolute correlation coefficient with SARS-CoV-2 VL in temporally aligned blood and swab samples. (D) Temporal kinetics of SARS-CoV-2 VL and genes showing moderate-to-strong associations with VL in previously vaccinated and unvaccinated COVID-19+ participants. Solid lines represent generalized additive model smoothing across all participants within a group, and colored shades represent the 95% confidence interval. Green, red, and blue colors denote healthy, COVID-19+ unvaccinated, and COVID-19+ vaccinated participants, respectively. Dotted lines represent individual temporal trajectories of gene. (E) Temporal kinetics of IFI27 gene expression, VL, and symptom burden in individual participants.
See this image and copyright information in PMC

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