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. 2023 Nov 8:8:507.
doi: 10.12688/wellcomeopenres.20259.1. eCollection 2023.

A chromosomal reference genome sequence for the malaria mosquito, Anopheles moucheti, Evans, 1925

Sandrine N Nsango  1 , Jean-Pierre Agbor  1 , Diego Ayala  2   3 , Harriet F Johnson  4 , Haynes Heaton  5 , Martin G Wagah  4 , Joanna C Collins  4 , Ksenia Krasheninnikova  4 , Sarah E Pelan  4 , Damon-Lee B Pointon  4 , Ying Sims  4 , James W Torrance  4 , Alan Tracey  4 , Marcela Uliano Da Silva  4 , Jonathan M D Wood  4 , Katharina von Wyschetzki  4 ; DNA Pipelines collective; Shane A McCarthy  4   6 , Daniel E Neafsey  7   8 , Alex Makunin  4 , Mara Lawniczak  4
Affiliations

A chromosomal reference genome sequence for the malaria mosquito, Anopheles moucheti, Evans, 1925

Sandrine N Nsango et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual male Anopheles moucheti (the malaria mosquito; Arthropoda; Insecta; Diptera; Culicidae), from a wild population in Cameroon. The genome sequence is 271 megabases in span. The majority of the assembly is scaffolded into three chromosomal pseudomolecules with the X sex chromosome assembled. The complete mitochondrial genome was also assembled and is 15.5 kilobases in length.

Keywords: African malaria mosquito; Anopheles moucheti; chromosomal; genome sequence.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Snail plot summary of assembly statistics for Anopheles moucheti assembly idAnoMoucSN_F20_07.
The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 271,332,225 bp assembly. The distribution of sequence lengths is shown in dark grey with the plot radius scaled to the longest sequence present in the assembly (103,511,506 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 sequence lengths (92,649,126 and 283,162 bp), respectively. The pale grey spiral shows the cumulative sequence count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the diptera_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/idAnoMoucSN_F20_07/dataset/CALSEA01/snail.
Figure 2.
Figure 2.. Blob plot of base coverage in idAnoMoucSN-F20_07 PacBio HiFi reads against GC proportion for An. moucheti assembly idAnoMoucSN_F20_07.
Chromosomes are coloured by phylum. Circles are sized in proportion to chromosome length. Histograms show the distribution of chromosome length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/idAnoMoucSN_F20_07/dataset/CALSEA01/blob.
Figure 3.
Figure 3.. Cumulative chromosome length for An. moucheti assembly idAnoMoucSN_F20_07.
The grey line shows cumulative length for all chromosomes. Coloured lines show cumulative lengths of chromosomes assigned to each phylum using the buscogenes taxrule. The interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/idAnoMoucSN_F20_07/dataset/CALSEA01/cumulative.
Figure 4.
Figure 4.. Genome assembly of An. moucheti, idAnoMoucSN_F20_07: Hi-C contact map.
Visualised in HiGlass. Chromosomes are arranged in size order from left to right and top to bottom. X chromosome signal is lower as individuals used for both PacBio and HiC are males. The interactive Hi-C map can be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=GZ6DShODRcSMNgw7a0UIfQ.
Figure 5.
Figure 5.. Distribution plot of repeat masked sequence proportion against genomic position for chromosomes of An. moucheti, idAnoMoucSN_F20_07.
1Mb windows with assembly gaps highlighted by larger blue circles, size is proportional to total size of gaps in the window (max 7800bp per window).
Figure 6.
Figure 6.. Alignment dotplot between genome assemblies of An. moucheti (idAnoMoucSN_F20_07) and An. gambiae, AgamP4 (PEST).
Visualised in DGenies. Chromosome arms correspondence (moucheti-gambiae): 2R-2R, 2L-3L, 3R-3R, 3L-2L in agreement with .
See this image and copyright information in PMC

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