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. 2024 Jan 17;13(1):e0081123.
doi: 10.1128/MRA.00811-23. Epub 2023 Dec 4.

Direct metagenomic and amplicon-based Nanopore sequencing of French human monkeypox from clinical specimen

Aurelia Kwasiborski  1 , Véronique Hourdel  1 , Charlotte Balière  1 , Damien Hoinard  1 , Quentin Grassin  1 , Maxence Feher  1 , Clémentine De La Porte Des Vaux  2 , Mélanie Cresta  2 , Jessica Vanhomwegen  1 , Jean-Claude Manuguerra  1 , Christophe Batéjat  1 , Valérie Caro  1
Affiliations

Direct metagenomic and amplicon-based Nanopore sequencing of French human monkeypox from clinical specimen

Aurelia Kwasiborski et al. Microbiol Resour Announc. .

Abstract

We report the whole-genome sequence of monkeypox virus obtained using MinION technology (Oxford Nanopore Technologies) from a French clinical specimen during the 2022 epidemic. Amplicon-based sequencing and shotgun metagenomic approaches were directly applied to the sample.

Keywords: amplicon-based sequencing; metagenomics; monkeypox virus; nanopore; next-generation sequencing.

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Conflict of interest statement

The authors declare no conflict of interest.

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