Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Dec 1;64(15):4.
doi: 10.1167/iovs.64.15.4.

Correlation Between Vitreous Level of Angiogenic Growth Factors and Oxygen Saturation in Retinal Vessels in Diabetic Retinopathy

Hana Morin  1 , Jan Havlík  1 , Oldrich Chrapek  2   3 , Michal Hrevuš  4 , Pavel Nemec  1 , Leoš Rejmont  1 , Jan Tesar  1 , Marta Kalousova  5 , Tomaš Zima  5 , Martin Šín  1
Affiliations

Correlation Between Vitreous Level of Angiogenic Growth Factors and Oxygen Saturation in Retinal Vessels in Diabetic Retinopathy

Hana Morin et al. Invest Ophthalmol Vis Sci. .

Abstract

Purpose: The aim of this study was to investigate whether there is any association between the levels of the angiogenic growth factors and the vascular oxygen saturation in eyes with diabetic retinopathy.

Methods: The study was designed as a prospective trial. The cohort consisted of 29 diabetic patients with scheduled vitreous procedures (intravitreal injection or pars plana vitrectomy). The control group included 30 patients scheduled for macular surgery (macular hole or epiretinal membrane). Nine patients (four from the diabetic maculopathy [DM] group and five from the control group) were excluded from the study because of unsuccessful vitreous samples. Retinal oximetry was performed several hours before the vitreous procedure was performed, and vitreous samples were obtained during the procedure. The concentrations of VEGF, Serpin F1/pigment epithelium-derived factor (PEDF), and placental growth factor (PlGF) were measured by ELISA.

Results: A negative correlation between level of VEGF and arteriovenous (AV) saturation difference was determined in the DM group (Pearson correlation coefficient r = -0.607; two-tailed test, P = 0.002). Also a negative correlation between level of PlGF and AV saturation difference was determined in the DM group (Pearson correlation coefficient r = -0.521; two-tailed test, P = 0.011) A positive correlation between PlGF level and the vein saturation was not statistically significant (Pearson correlation coefficient r = 0.325; two-tailed test, P = 0.130). We did not find any correlation between vitreous level of PEDF and vascular saturation within the DM group.

Conclusions: Our findings in diabetic patients suggests a correlation between the intravitreal level of proangiogenic factors and the AV difference measured by retinal oximetry.

PubMed Disclaimer

Conflict of interest statement

Disclosure: H. Morin, None; J. Havlík, None; O. Chrapek, None; M. Hrevuš, None; P. Němec, None; L. Rejmont, None; J. Tesař, None; M. Kalousova, None; T. Zima, None; M. Šín, None

Figures

Figure 1.
Figure 1.
An oximetry image. The two blue circles demarcate the measurement area. The inner circle has a diameter of 1.5 disc diameter. The outer circle has a diameter of 3.0 disc diameters. The main retinal vessel segments in this area are chosen according to a set of detailed rules (Oxymap protocol from November 21, 2013, simple means used).
Figure 2.
Figure 2.
Bar charts of retinal vessel oxygen saturation values in the different study groups.
Figure 3.
Figure 3.
Correlation between AV difference (%) and vitreous level of VEGF (pg/mL). Pearson’s correlation coefficient r = −0.607; two-tailed test, P = 0.002.
Figure 4.
Figure 4.
Correlation between AV difference (%) and vitreous level of PlGF (pg/mL). Pearson’s correlation coefficient r = −0.521; two-tailed test, P = 0.011.
Figure 5.
Figure 5.
Correlation between AV difference (%) and log VEGF values. Pearson’s correlation coefficient r = −0.821; two-tailed test, P < 0.00001.
See this image and copyright information in PMC

References

    1. Hammer M, Vilser W, Riemer T, et al. .. Diabetic patients with retinopathy show increased retinal venous oxygen saturation. Graefes Arch Clin Exp Ophthalmol. 2009; 247: 1025–1030. - PubMed
    1. Hardarson SH, Stefánsson E.. Retinal oxygen saturation is altered in diabetic retinopathy. Br J Ophthalmol. 2012; 96: 560–563. - PubMed
    1. Khoobehi B, Firn K, Thompson H, Reinoso M, Beach J.. Retinal arterial and venous oxygen saturation is altered in diabetic patients. Invest Ophthalmol Vis Sci. 2013; 54: 7103. - PubMed
    1. Jørgensen CM, Hardarson SH, Bek T.. The oxygen saturation in retinal vessels from diabetic patients depends on the severity and type of vision-threatening retinopathy. Acta Ophthalmol (Copenh). 2014; 92: 34–39. - PubMed
    1. Šínová I, Chrapek O, Mlčák P, Řehák J, Karhanová M, Šín M. Automatic retinal oxymetry in patients with diabetic retinopathy. Czech Slovak Ophthalmol. 2016; 72: 182–186. - PubMed

Publication types

Substances