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. 2025 Jan 1;64(1):126-132.
doi: 10.1093/rheumatology/kead641.

Serum calprotectin correlates stronger with inflammation and disease activity in ACPA positive than ACPA negative rheumatoid arthritis

Kristina Sejersen  1   2 , Tomas Weitoft  3 , Ann Knight  4 , Jörgen Lysholm  5 , Anders Larsson  1 , Johan Rönnelid  6
Affiliations

Serum calprotectin correlates stronger with inflammation and disease activity in ACPA positive than ACPA negative rheumatoid arthritis

Kristina Sejersen et al. Rheumatology (Oxford). .

Abstract

Objectives: The aim of the present study was to evaluate the performance of serum and SF levels of the granulocyte protein calprotectin as an inflammatory biomarker in RA patients with knee synovitis.

Methods: Seventy-six RA patients with ongoing knee synovitis were included. Data on DAS with 28 joints and their subcomponents and radiological destruction of the affected knee were collected. White blood cell count, CRP, ACPA against cyclic citrullinated peptide version 2 (anti-CCP2), IgM RF and calprotectin were analysed in parallel in circulation and in SF. Counts of polynuclear and mononuclear cells were measured in SF.

Results: Serum (S)-calprotectin correlated more strongly than SF-calprotectin with inflammatory markers and disease activity. Instead, SF-calprotectin showed a strong correlation to SF counts of white blood cells, and especially to polymorphonuclear cell counts (Spearman's ρ = 0.72, P < 0.001). S-calprotectin showed markedly stronger correlation with inflammatory markers and disease activity in ACPA positive as compared with ACPA negative RA patients; a similar difference was observed for patients with and without IgM RF.

Conclusion: The particularly strong association between circulating calprotectin and inflammation in ACPA positive RA is a new argument for a specific role for polymorphonuclear granulocytes/neutrophils in this RA subset. Measurement of calprotectin in SF does not convey any additional benefit compared with measurement in the circulation in RA patients with knee synovitis.

Keywords: anti-citrullinated peptide antibodies; antibodies against cyclic citrullinated peptide version 2; calprotectin; inflammation; rheumatoid arthritis; rheumatoid factor.

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References

    1. Smolen JS, Aletaha D, McInnes IB.. Rheumatoid arthritis. Lancet 2016;388:2023–38. - PubMed
    1. Zhang L, Yuan Y, Xu Q, Jiang Z, Chu CQ.. Contribution of neutrophils in the pathogenesis of rheumatoid arthritis. J Biomed Res 2019;34:86–93. - PMC - PubMed
    1. Schenten V, Bréchard S, Plançon S. et al. iPLA2, a novel determinant in Ca2+- and phosphorylation-dependent S100A8/A9 regulated NOX2 activity. Biochim Biophys Acta 2010;1803:840–7. Erratum in: Biochim Biophys Acta 2011;1813(12):2196. - PubMed
    1. Fagerhol MK, Dale I, Andersson T.. A radioimmunoassay for a granulocyte protein as a marker in studies on the turnover of such cells. Bull Eur Physiopathol Respir 1980;16(Suppl):273–82. - PubMed
    1. Edgeworth J, Gorman M, Bennett R. et al. Identification of p8,14 as a highly abundant heterodimeric calcium binding protein complex of myeloid cells. J Biol Chem 1991;266:7706–13. - PubMed

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