Identification of a new protein synthesis initiation factor from wheat germ
- PMID: 3804998
Item in Clipboard
Identification of a new protein synthesis initiation factor from wheat germ
J Biol Chem.
.
Free article
Abstract
A previously unidentified factor has been isolated from wheat germ that stimulates globin mRNA-directed polypeptide synthesis in vitro. This factor is separated from eukaryotic initiation factor (eIF)-4B by chromatography on m7GTP-Sepharose. eIF-4B binds to m7GTP-Sepharose, whereas the stimulatory factor does not. Further purification of the factor yields a preparation that contains one major polypeptide with a molecular weight of approximately 59,000, This factor enhances the binding of globin mRNA to 40 S ribosomal subunits in the presence of eIF-2, eIF-3, eIF-4A, and either eIF-4B or eIF-4F and has been designated eIF-4G.
Similar articles
-
Evidence that the 59-kDa protein synthesis initiation factor from wheat germ is functionally similar to the 80-kDa initiation factor 4B from mammalian cells.J Biol Chem. 1989 May 25;264(15):8491-4. J Biol Chem. 1989. PMID: 2722784
-
Identification of an isozyme form of protein synthesis initiation factor 4F in plants.J Biol Chem. 1992 May 15;267(14):10096-100. J Biol Chem. 1992. PMID: 1577779
-
Translation initiation factors that function as RNA helicases from mammals, plants and yeast.Biochim Biophys Acta. 1990 Aug 27;1050(1-3):134-9. doi: 10.1016/0167-4781(90)90154-t. Biochim Biophys Acta. 1990. PMID: 2169888
-
Purification and properties of protein synthesis initiation and elongation factors from wheat germ.Methods Enzymol. 1986;118:109-28. doi: 10.1016/0076-6879(86)18068-2. Methods Enzymol. 1986. PMID: 3512960 Review. No abstract available.
-
Regulation of eukaryotic protein synthesis by initiation factors.J Biol Chem. 1993 Feb 15;268(5):3017-20. J Biol Chem. 1993. PMID: 8428975 Review. No abstract available.
Cited by
-
Serum-stimulated, rapamycin-sensitive phosphorylation sites in the eukaryotic translation initiation factor 4GI.EMBO J. 2000 Feb 1;19(3):434-44. doi: 10.1093/emboj/19.3.434. EMBO J. 2000. PMID: 10654941 Free PMC article.
-
The plant translational apparatus.Plant Mol Biol. 1996 Oct;32(1-2):107-44. doi: 10.1007/BF00039380. Plant Mol Biol. 1996. PMID: 8980477 Review.
-
Affinity resins containing enzymatically resistant mRNA cap analogs--a new tool for the analysis of cap-binding proteins.RNA. 2012 Jul;18(7):1421-32. doi: 10.1261/rna.032078.111. Epub 2012 May 15. RNA. 2012. PMID: 22589334 Free PMC article.
-
Mechanism and regulation of eukaryotic protein synthesis.Microbiol Rev. 1992 Jun;56(2):291-315. doi: 10.1128/mr.56.2.291-315.1992. Microbiol Rev. 1992. PMID: 1620067 Free PMC article. Review.
-
Cap-independent translation conferred by the 5' leader of tobacco etch virus is eukaryotic initiation factor 4G dependent.J Virol. 2001 Dec;75(24):12141-52. doi: 10.1128/JVI.75.24.12141-12152.2001. J Virol. 2001. PMID: 11711605 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous
