Immunological evidence for gap junction polypeptide in plant cells

Abstract

A whole cell homogenate prepared from soybean (Glycine max (L.) Merr. cv. Mandarin) root cells (SB-1 cell line) was electrophoresed on a sodium dodecyl sulfate-polyacrylamide gel and transferred to nitrocellulose paper. The nitrocellulose was probed with a monospecific antibody capable of recognizing the Mr 27,000 polypeptide of rat liver gap junctions; this antibody was prepared from immune serum raised against gap junctions purified from V79 cells (Chinese lung fibroblasts). The immunoblots afforded two polypeptides migrating at Mr 29,000 and 48,000. This pattern of blotting was also observed when homogenates of soybean or poinsettia leaves excised from whole plants were probed with anti-V79 gap junction antiserum. Gap junction purification schemes, developed for rat liver (Hertzberg, E. L. (1984) J. Biol. Chem. 259, 9936-9943), were employed on soybean protoplast homogenates yielding a significant enrichment for the Mr 29,000 and 48,000 polypeptides as judged by Coomassie Blue staining and immunoblotting with anti-V79 gap junction antiserum. These immunological results provide the first reported evidence for a homologous gap junction polypeptide in plant cells.