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. 2023 Dec 4;46(4):e20230015.
doi: 10.1590/1678-4685-GMB-2023-0015. eCollection 2023.

Characterization of a new anellovirus species infecting an ocelot (Leopardus pardalis) in Brazil

Liliane T F Cavalcante  1 , Matheus A C Cosentino  1 , Mirela D'arc  1 , Filipe R R Moreira  1 , Ricardo Mouta  1 , Anderson M Augusto  2 , Fernando Troccoli  2 , Marcelo A Soares  1   3 , André F Santos  1
Affiliations

Characterization of a new anellovirus species infecting an ocelot (Leopardus pardalis) in Brazil

Liliane T F Cavalcante et al. Genet Mol Biol. .

Abstract

A complete genome of the first anellovirus infecting the wild felid Leopardus pardalis (ocelot) and a partial genome were assembled and annotated through high-throughput sequencing protocols followed by Sanger sequencing validation. The full-length virus obtained comprises 2,003 bp, while the partial genome comprises 1,224 bp. Phylogenetic analysis grouped these two sequences in two distinct clusters related to previously described Felidae anelloviruses. The ORF1 of the partial genome was identified as a new species provisionally called Torque teno ocelot virus, with 53.6% identity with its sister lineage. The complete genome was inferred as a new representative of the Torque teno felid virus 3 species, with 73.28% identity to the closest reference. This study expands known virus diversity and the host span of anelloviruses.

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Conflict of interest statement

Conflict of Interest: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be defined as a conflict of interest.

Figures

Figure 1 -
Figure 1 -. Maximum likelihood phylogeny of the ORF1 gene of the Anelloviridae family. Phylogeny of the ORF1 gene, inferred with a dataset of an alignment of 1183 aa length and 884 sequences under the LG+F+G4 model. The tree was midpoint-rooted for visualization purposes. Node shapes colored in black represent node support for SH-aLRT and Ultrafast Bootstrap equal or superior to 75. When only SH-aLRT was superior to this threshold, node shapes were represented in gray. Conversely, when only Ultrafast Bootstrap was superior, the white color was used. Nodes with support inferior to 75 in both parameters were not marked. Tip labels are colored according to the identified host, where orange tip labels represent anelloviruses identified in Puma concolor, dark yellow in Leopardus pardalis, gray in Felis catus and light yellow in other Felidae. (A) Maximum likelihood phylogeny of the ORF1 gene of the Anelloviridae family, with clades highlighted in yellow to evidence Leopardus pardalis anellovirus. (B) Monophyletic clade of Torque teno felid virus 3 genomes identified in L. pardalis. (C) Monophyletic clade of the novel putative species Torque teno ocelot virus.
See this image and copyright information in PMC

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