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. 2023 Dec 1:38:e386723.
doi: 10.1590/acb386723. eCollection 2023.

Lacticaseibacillus casei decrease long-chain fatty acids and most substances in an experimental model of intestinal mucositis

Stphannie Jamyla de Araújo Barbosa  1 , Amanda Silveira da Silva  2 , Maisie Mitchele Barbosa Oliveira  3 , Susana Barbosa Ribeiro  3 , Caroline Addison Carvalho Xavier de Medeiros  3 , Leandro de Santis Ferreira  3 , Francisco Ayrton Senna Domingos Pinheiro  3 , Francisco Canindé de Sousa Junior  1 , Agnes Andrade Martins  4 , Raimundo Fernandes de Araújo Júnior  5 , Vinícius Barreto Garcia  5 , Aurigena Antunes de Araújo  4
Affiliations

Lacticaseibacillus casei decrease long-chain fatty acids and most substances in an experimental model of intestinal mucositis

Stphannie Jamyla de Araújo Barbosa et al. Acta Cir Bras. .

Abstract

Purpose: To evaluate the long-chain fatty acid and major compounds levels in the feces after prophylactic oral use of Lacticaseibacillus casei in an experimental model of intestinal mucositis.

Methods: Fifteen Swiss mice were randomly divided into three groups (n=5/group): The negative or positive control groups (n = 5) received saline orally for 18 days and an the intraperitoneal (i.p.) of saline or 5 Fluorouracil (450 mg/kg) in 15th day, respectability. L. casei group received oral concentration of L. casei (1x109 CFU/mL) for 18 days, the i.p. injection of 5-fluorouracil (450 mg/kg) in 15th days. Tissue samples from colon and each small intestine segment were collected for histopathological analysis. Stool samples were collected. Fecal composition of long-chain fatty acids and sterols were analysed by gas chromatography-mass spectrometry on the 15th and the 18th day.

Results: The mucosa layer of all small intestine segments of animals from L. casei showed well preserved epithelium and glands, without necrosis signs, but Goblet cells number decreased. Several long-chain fatty acids and sterols have been identified before and after in the groups. L. casei administration after 5-FU treatment reduced concentrations of linoleic acid (18:2) (p < 0.001) and oleic acid (18:1) (p < 0.001) in feces.

Conclusions: L. casei prevented the mucosal damage associated with 5-FU-induced intestinal mucositis reduced long-chain fatty acid levels in the feces.

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Conflict of interest statement

Conflict of interest: Nothing to declare.

Figures

Figure 1
Figure 1. Description of histological characteristics of duodenum, jejunum, ilium and colon in CN, CP and L. casei group. CN: Histopathological of duodenum, jejunum, ileum and colon shows well preserved mucosa, with long villi (black arrows) and well preserved crypts. The submucosa is intact, with normal submucosal glands and no signs of inflammatory infiltration (yellow arrow) or necrosis (green arrow). Several goblet cells were found (red arrow), especially in the Colon. CP: 5-fluorouracil (5-FU)-injury group showed clear signs of damage, with short villi and small crypts and little evidence of goblet cells in duodenum’s mucosa. The submucosa is also injured, with signs of inflammatory infiltration and few submucosal glands. Both jejunum and ileum’s mucosa showed shortened villi and poorly preserved crypts in 5-FU-injured group, and just a few goblet cells were found in jejunum. Submucosal jejunum layer was heavily infiltrated by inflammatory cells (yellow arrow), with some signs of necrosis (green arrow). Short crypts were found in the colon’s mucosa, as well as necrosis foci. L. casei: no sign of inflammatory infiltrate was found in the submucosa layer of the colon. L. casei protected the colon and some small intestine segments against 5-FU-induced injury. Although inflammatory infiltration was still present throughout the whole small intestine submucosal layer (yellow arrow), the mucosa layer of all small intestine segments showed well preserved epithelium and glands, without necrosis signs. Goblet cells number decreased. No signs of injury were found in the colon segments.
Figure 2
Figure 2. Gas chromatography mass spectrometry analysis identified the composition of long-chain fatty acids day 18 for the negative control (CN), positive control (CP) and Lacticaseibacillus casei groups. Lacticaseibacillus casei decrease oleic acid (p < 0.001) and linoleic acid (p < 0.001) after induction of intestinal mucositis by 5-fluorouracil.
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