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. 2024 Feb;598(3):302-320.
doi: 10.1002/1873-3468.14781. Epub 2023 Dec 14.

CRK and NCK adaptors may functionally overlap in zebrafish neurodevelopment, as indicated by common binding partners and overlapping expression patterns

Helaina R Stergas  1 , Maeve Dillon-Martin  1 , Caroline M Dumas  1 , Nicole A Hansen  1 , Francesca J Carasi-Schwartz  1 , Alex R D'Amico  1 , Kylie M Finnegan  1 , Uatchet Juch  1 , Keeley R Kane  1 , Isabel E Kaplan  1 , Madeline L Masengarb  1 , Marina E Melero  1 , Lauren E Meyer  1 , Conrad R Sacher  1 , Evan A Scriven  1 , Alicia M Ebert  1 , Bryan A Ballif  1
Affiliations

CRK and NCK adaptors may functionally overlap in zebrafish neurodevelopment, as indicated by common binding partners and overlapping expression patterns

Helaina R Stergas et al. FEBS Lett. 2024 Feb.

Abstract

CRK adaptor proteins are important for signal transduction mechanisms driving cell proliferation and positioning during vertebrate central nervous system development. Zebrafish lacking both CRK family members exhibit small, disorganized retinas with 50% penetrance. The goal of this study was to determine whether another adaptor protein might functionally compensate for the loss of CRK adaptors. Expression patterns in developing zebrafish, and bioinformatic analyses of the motifs recognized by their SH2 and SH3 domains, suggest NCK adaptors are well-positioned to compensate for loss of CRK adaptors. In support of this hypothesis, proteomic analyses found CRK and NCK adaptors share overlapping interacting partners including known regulators of cell adhesion and migration, suggesting their functional intersection in neurodevelopment.

Keywords: functional compensation; proteomics; signal transduction; zebrafish eye development.

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References

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