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. 2024 Jan 11;12(1):e0317923.
doi: 10.1128/spectrum.03179-23. Epub 2023 Dec 7.

Evaluation of mycobacteria infection prevalence and optimization of the identification process in North Sardinia, Italy

Xiang Chen  1   2 , Leonardo Antonio Sechi  1   3 , Paola Molicotti  1   3
Affiliations

Evaluation of mycobacteria infection prevalence and optimization of the identification process in North Sardinia, Italy

Xiang Chen et al. Microbiol Spectr. .

Abstract

Mycobacterial infection is a major threat to public health worldwide. Accurate identification of infected species and drug resistance detection are critical factors in treatment. We focused on shortening the turn-around time of identifying mycobacteria species and antibiotic resistance tests.

Keywords: Mycobacterium tuberculosis; drug resistance; identification process; non-tuberculous mycobacteria.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
Flowchart of mycobacteria identification.
Fig 2
Fig 2
MTB-positive samples detected in different types of specimens.
Fig 3
Fig 3
Antibiotic susceptibility test results of MTB-positive samples.
Fig 4
Fig 4
Comparison of DNA probe and MALDI results. Location of probes: 1. FN, false negative; 2. MYC genus, Mycobacterium; 3. MTB, M. tuberculosis complex; 4. MKA-1, M. kansasii (group I); 5. MKA-2, M. kansasii (group II); 6. MKA-3, M. kansasii (groups III, IV, and V) and Mycobacterium gastri; 7. MXE, M. xenopi; 8. MGO, M. gordonae; 9. MGV, M. genavense; 10. MSI, Mycobacterium simiae; 11. MMU, Mycobacterium marinum and Mycobacterium ulcerans; 12. MCE, Mycobacterium celatum; 13. MAIS, M. avium, M. intracellulare, Mycobacterium scrofulaceum, and Mycobacterium malmoense; 14. MAV, M. avium, Mycobacterium paratuberculosis, and Mycobacterium silvaticum; 15. MIN-1, M. intracellulare (sequevars Min-A, -B, -C, and -D); 16. MIN-2, M. intracellulare (sequevar Mac-A); 17. MSC, M. scrofulaceum; 18. MML, M. malmoense; 19. MHP, Mycobacterium haemophilum; 20. MCH-1, M. chelonae (groups I, II, III, and IV); 21. MCH-2, M. chelonae (group III); 22. MCH-3, M. chelonae (group I); 23. MFO, M. fortuitum; and 24. MSM, Mycobacterium smegmatis.
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