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. 2024 Feb;46(1):55-68.
doi: 10.1007/s10529-023-03448-x. Epub 2023 Dec 8.

Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production

Anqi Zhao  1 , Yamei Li  1 , Lixia Wu  1 , Zhi Wang  2 , Yongkun Lv  2 , Wenlong Xiong  2 , Mohammad Asraful Alam  2 , Guohua Liu  3 , Jingliang Xu  4
Affiliations

Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production

Anqi Zhao et al. Biotechnol Lett. 2024 Feb.

Abstract

Objectives: Enhance the androstadienedione (Androst-1,4-diene-3,17-dione, ADD) production of rough morphotype Mycolicibacterium neoaurum R by repeated-batch fermentation of immobilized cells.

Results: M. neoaurum R was a rough colony morphotype variant, obtained from the routine plating of smooth M. neoaurum strain CICC 21097. M. neoaurum R showed rougher cell surface and aggregated in broth. The ADD production of M. neoaurum R was notably lower than that of M. neoaurum CICC 21097 during the free cell fermentation, but the yield gap could be erased after proper cell immobilization. Subsequently, repeated-batch fermentation of immobilized M. neoaurum R was performed to shorten the production cycle and enhance the bio-production efficiency of ADD. Through the optimization of the immobilization carriers and the co-solvents for phytosterols, the ADD productivity of M. neoaurum R immobilized by semi-expanded perlite reached 0.075 g/L/h during the repeated-batch fermentation for 40 days.

Conclusions: The ADD production of the rough-type M. neoaurum R was notably enhanced by the immobilization onto semi-expanded perlite. Moreover, the ADD batch yields of M. neoaurum R immobilized by semi-expanded perlite were maintained at high levels during the repeated-batch fermentation.

Keywords: Androst-1,4-diene-3,17-dione; Cell immobilization; Mycolicibacterium; Perlite; Rough colony morphotype variant.

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